NDLI: Integrated bioanalytical microsystem

Content Provider	IEEE Xplore Digital Library
Author	I-Ming Hsing Hong Cai
Copyright Year	2004
Description	Author affiliation: Dept. of Chem. Eng., Hong Kong Univ. of Sci. & Technol., China (I-Ming Hsing; Hong Cai)
Abstract	A newly developed integrated bio-microsystem that allows for sequence-specific DNA analysis is presented. Micro-fabricated Si/glass-based devices with functionalities of simultaneous polymerase chain reaction (PCR) target amplification and sequence-specific electrochemical (EC) detection have been successfully developed. The microdevice consists of a reaction chamber formed in a silicon substrate, and an EC sensor fabricated onto a glass substrate. Heaters and temperature sensors are patterned on top of the reaction chamber for thermal cycling of PCR. The glass substrate serves not only as a seal for the reaction chamber but also as a platform for the EC detection of the PCR product. Both the amplification of the target DNA sequence and the subsequent EC detection of the PCR product are carried out inside the reaction chamber. Sequence-specific recognition of the target analyte is achieved through hybridization with the probe immobilized onto the working electrode on the glass substrate. Technical challenges of this PCR-EC microdevice and its application to multiplex pathogen identification are elucidated.
Starting Page	1767
Ending Page	1772
File Size	899224
Page Count	6
File Format	PDF
ISBN	078038511X
DOI	10.1109/ICSICT.2004.1435175
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2004-10-18
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Glass DNA Polymers Silicon Biosensors Temperature sensors Seals Sequences Target recognition Probes
Content Type	Text
Resource Type	Article

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