NDLI: Optimization of the pulsed electric field decontamination method in continous flow

Content Provider	IEEE Xplore Digital Library
Author	Gusbeth, C.A. Frey, W. Strassner, R. Schwartz, T. Rieder, A.
Copyright Year	2008
Description	Author affiliation: Inst. fur Hochleistungsimpuls- und Mikrowellentech. (IHM), Forschungszentrum Karlsruhe GmbH, Karlsruhe (Gusbeth, C.A.; Frey, W.; Strassner, R.)
Abstract	Summary form only given. Wastewater from hospitals carries large number of pathogenic and increasingly antibiotic resistant bacteria, which could spread in the environment. The pulsed electric field (PEF) treatment of wastewater as a non-chemical disinfection method is a suitable alternative method for reducing this bacterial freight. In the literature it was figured out, that continuous flow PEF treatment reduces more effectively bacterial contamination than batch reactor treatment. One explanation is that the heat generated during the PEF treatment in a continuous flow reactor is positively affecting the bacterial reduction. Depending on the PEF treatment energy, outlet temperatures of more than 70degC were achieved. Consequently, bacterial reduction depends on the initial wastewater temperature and on the electrical dissipated treatment energy. In our study we analysed the bacterial reduction depending on temperature. Filtered wastewater samples (from a wastewater purification plant) were inoculated with the (BRE; Strain 211, Mainz) and exposed to different PEF treatments at 40, 50 and 60degC. The lethal treatment temperature of the by heat treatment over 3-4 min is around 56degC. Treatment with 60degC for the same duration provokes a bacterial reduction of 4 log. Simultaneous treatments of inoculated wastewater samples with PEF and heating (40, 50 and 60degC) reduced the bacterial contamination over the expected effect, calculated as a sum of effects induced from both treatments. This is an indication for a synergistic effect induced through the simultaneous treatment with PEF and heat. In all experiments the specific energy was kept constant by adjusting the number of pulses. At 2degC, the bacterial reduction saturates already for PEF treatments with specific energies higher than 120 J/ml. At higher temperatures (over 40degC), the bacterial reduction increased until the complete reduction (over 8 log) was achieved at 60degC. To separate the thermal effect from the PEF effect, samples were treated first with PEF in a batch chamber and afterwards, about 30 s later, by heating over 4 min. No significant variation of the efficiency of bacterial reduction compared to the simultaneous treatment was found. These results show that the duration of cell membrane damage, is far longer as the assumed healing time (mus-range). This finding can be used for further optimization of the PEF treatment in continuous flow.
Starting Page	1
Ending Page	1
File Size	66650
Page Count	1
File Format	PDF
ISBN	9781424419296
ISSN	07309244
DOI	10.1109/PLASMA.2008.4590823
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-06-15
Publisher Place	Germany
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Microorganisms Wastewater Heat treatment Electric fields Temperature dependence Inductors Contamination
Content Type	Text
Resource Type	Article
Subject	Atomic and Molecular Physics, and Optics Condensed Matter Physics Electrical and Electronic Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in