NDLI: Effects of Mechanical Strain on Differentiation of Osteoblastic Cells

Content Provider	IEEE Xplore Digital Library
Author	Matsui, A. Masuda, T. Anada, T. Takahashi, I. Arai, F. Takami, M. Kamijo, R. Fukuda, T. Echigo, S. Suzuki, O.
Copyright Year	2008
Description	Author affiliation: Grad. Sch. of Eng., Tohoku Univ., Sendai (Arai, F.) \|\| Sch. of Dentistry, Showa Univ., Tokyo (Takami, M.; Kamijo, R.) \|\| Grad. Sch. of Eng. Furo-cho, Nagoya Univ., Nagoya (Fukuda, T.) \|\| Grad. Sch. of Dentistry, Tohoku Univ., Sendai (Matsui, A.; Masuda, T.; Anada, T.; Takahashi, I.; Echigo, S.; Suzuki, O.)
Abstract	The present study was designed to investigate whether compressive mechanical strain affects osteoblastic cell differentiation. We developed a culture system with the multi channel that induces the mechanical stimuli with quantitative gradient strain on seeded cells during the culture. This culture system was a computer-driven device that the various mode of mechanical strains can be applied, using the pressurization or depressurization, to deform cells cultured on PDMS membrane. The mRNA expression levels of osteoblast-related genes, such as osterix, collagen type I, and osteopontin, determined by real-time PCR, decreased by the compressive strain. On the contrary, the expression of RANKL, a factor related to the induction of osteoclasts, increased in the compressive strain, while the expression of osteoprotegrin, a molecule that antagonizes the function of the RANKL, decreased in the same strain. It was suggested that the function of osteoblastic cells may be influenced by the compressive strain.
Starting Page	475
Ending Page	480
File Size	1684091
Page Count	6
File Format	PDF
ISBN	9781424429189
DOI	10.1109/MHS.2008.4752499
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-11-06
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Capacitive sensors Tensile strain Biomembranes Design engineering Dentistry Biochemistry Strain control Compressive stress Tensile stress Bones
Content Type	Text
Resource Type	Article

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