NDLI: Nano-Scale Observation of Higher-Order Genome Structure Reconstituted from DNA and Chromosome Proteins

Content Provider	IEEE Xplore Digital Library
Author	Hizume, K. Sumiko, A. Yoshikawa, K. Takeyasu, K. Yoshimura, S.H.
Copyright Year	2008
Description	Author affiliation: Grad. Sch. of Biostudies, Kyoto Univ., Kyoto (Hizume, K.; Takeyasu, K.; Yoshimura, S.H.) \|\| Dept. of Phys., Kyoto Univ., Kyoto (Sumiko, A.; Yoshikawa, K.)
Abstract	In order to analyze chromatin higher-order structures, we have developed an experimental system to assemble long nucleosomal fibers and linker histone H1-induced thicker fibers from DNA and purified histones. We reconstituted chromatin fibers using normal, tailless, and hyper-acetylated core histones to reveal the effect of histone tail on the higher-order assembly of nucleosomes. AFM observation of these nucleosomes revealed that the interaction between nucleosomes decreased both by hyper-acetylation and the removal of the tails. The formation of H1-induced thick fiber was also affected by the acetylation and the tail-removal. The nucleosomes with hyper-acetylated core histones formed a thinner fiber, and the tailless nucleosome could not form any further fibrous structures by H1. These results suggested that the histone tail has a critical role in nucleosome-nucleosome interaction, which is required for the formation of H1-induced 30-nm fibers.
Starting Page	65
Ending Page	70
File Size	3722653
Page Count	6
File Format	PDF
ISBN	9781424429189
DOI	10.1109/MHS.2008.4752424
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-11-06
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Genomics Bioinformatics DNA Biological cells Tail Protein engineering Assembly systems Wrapping Laboratories Nuclear and plasma sciences
Content Type	Text
Resource Type	Article

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