NDLI: On-Chip Temperature Sensing and Control for Cell Immobilization and Culture System

Content Provider	IEEE Xplore Digital Library
Author	Yamanishi, Y. Chow, E. Teramoto, J. Magariyama, Y. Ishihama, A. Fukuda, T. Arai, F.
Copyright Year	2008
Description	Author affiliation: Dept. of Micro-Nano Syst. Eng., Nagoya Univ., Nagoya (Magariyama, Y.; Fukuda, T.) \|\| Dept. of Bioeng. & Robot., Tohoku Univ., Sendai (Yamanishi, Y.; Chow, E.; Arai, F.) \|\| Nat. Agric. & Food Res. Organ., Tsukuba (Teramoto, J.; Ishihama, A.)
Abstract	This paper presents a temperature sensing and controlling microfluid chip for cell immobilization using a thermo-sensitive hydrogel (PNIPAAm). The ITO (Indium Tin Oxide) microheaters fabricated by micromachining technology, perform heating of the solution of cell, PNIPAAm and Calcein-AM (fluorescent dye) in microchannels. It is important to note that a thick gel formation cause to block the observation of culturing cells due to the autofluorescence of gel which reduce the S/N ratio. For the present study, we are targeting to fabricate a suspended micro-bridge above a microheater to limit the height of gel to form "thin and transparent gel layer" above the heater. The research was focused on controlling of gel thickness which eliminated unnecessary interferences of autofluorescence of gel in the observation by the inverted microscope, and a better S/N ratio can be obtained than the conventional observation with uncontrolled gel thickness. All the heating and a suspended biocompatible micro-bridge were integrated on a chip, in which yeast cells immobilization can be performed by the gelation of the PNIPAAm solution.
Starting Page	295
Ending Page	300
File Size	6310711
Page Count	6
File Format	PDF
ISBN	9781424429189
DOI	10.1109/MHS.2008.4752466
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-11-06
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	System-on-a-chip Temperature sensors Temperature control Control systems Indium tin oxide Heating Micromachining Fluorescence Microchannel Thickness control
Content Type	Text
Resource Type	Article

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