NDLI: Reconstitution and Microscopic Observation of G Protein Subunits on Giant Liposomes: Attempt to Construct a Cell Model with Functional Membrane Protein Components

Content Provider	IEEE Xplore Digital Library
Author	Tsumoto, K. Yamazaki, Y. Kamiya, K. Yoshimura, T.
Copyright Year	2008
Description	Author affiliation: Dept. of Chem. for Mater., Mie Univ., Tsu (Tsumoto, K.; Yamazaki, Y.; Kamiya, K.) \|\| Dept. of Liposome Bioeng., Mie Univ., Tsu (Yoshimura, T.)
Abstract	Giant liposomes are cell-size artificial vesicles with phospholipid bilayers. Here, in order to obtain giant liposomes containing membrane proteins (giant proteoliposomes), we have introduced a novel procedure using membrane fusion phenomena between liposomes and recombinant baculovirus. We tried to thereby reconstitute heterotrimeric guanine nucleotide-binding protein (G protein) that is a peripheral membrane protein, i.e., Gs protein consisting of $alpha_{s},$ szlig and gamma, in giant proteoliposomes. Western blot analysis showed that expression of these proteins on both recombinant baculovirus budded virus envelopes and liposomes fused with the recombinant virus. Moreover, by using laser-scanning fluorescence microscopy, we observed that GTP analogues specifically bound to Gs alpha subunits on giant liposomes. These results indicate that using the present procedure giant proteoliposomes are prepared with successfully reconstituted G proteins.
Starting Page	145
Ending Page	150
File Size	792197
Page Count	6
File Format	PDF
ISBN	9781424429189
DOI	10.1109/MHS.2008.4752439
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-11-06
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Microscopy Biomembranes Protein engineering Cells (biology) Biological materials Biological system modeling Gene expression Lipidomics Chemistry Biomedical engineering
Content Type	Text
Resource Type	Article

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