NDLI: Broad substrate RNA specificity of Trm1 (tRNA $(m^{2}2G26)$ methyltransferase) from Aquifex aeolicus

Content Provider	IEEE Xplore Digital Library
Author	Awai, T. Hori, H.
Copyright Year	2008
Description	Author affiliation: Dept. of Mater. Sci. & Biotechnol., Ehime Univ., Matsuyama (Awai, T.; Hori, H.)
Abstract	RNA modification is one of the most fundamental biological phenomena because it is essential for cell viability through the regulation of protein synthesis. At present, 107 types of RNA modifications are reported. Among them, $N^{2},$ $N^{2}-dimethylguanosine$ at position 26 $(m^{2}$ $_{2}G26)$ is found in a half of tRNA species from archaea and 2/3 of tRNA species from eukaryotes. This modification is brought by tRNA $(m^{2}$ $_{2}G26)$ methyltransferase. This enzyme catalyzes the methyl-transfers from S-adenosyl-L-methionine to $N^{2}$ atom of the G26. In the reaction, two molecules of S-adenosyl-L-metnionine are consumed and $N^{2}-methylguanine$ at position 26 $(m^{2}G26)$ is generated as an intermediate. A hyper-thermophilic eubacterium, Aquifex aeolicus is supposed to evolutionarily diverge at the earliest period from the other bacteria as judged by the phylogenetic analyses of small rRNA and translation factors. In 1998, genome sequencing project has been reported that a putative trm1 gene is encoded in A. aeolicus genome. In general, the trm1 gene encodes tRNA $(m^{2}$ $_{2}G26)$ methyltransferase. At present, trm1 gene is not discovered from eubacteria except for A. aeolicus. To clarify whether this putative trm1 gene is encoded tRNA $(m^{2}$ $_{2}G26)$ methyltransferase or not, we constructed Trm1 overexpression system in E. coli and demonstrated that this trm1 gene product possesses a tRNA $(m^{2}$ $_{2}G26)$ methyltransferase activity. In this meeting, we discuss the substrate RNA specificity of the A. aeolicus Trm1.
Starting Page	163
Ending Page	168
File Size	5517472
Page Count	6
File Format	PDF
ISBN	9781424429189
DOI	10.1109/MHS.2008.4752442
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-11-06
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	RNA Genomics Bioinformatics Biological information theory Cells (biology) Proteins Archaea Biochemistry Microorganisms Phylogeny
Content Type	Text
Resource Type	Article

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