NDLI: Diamond photoluminescent nanoparticles as innovative markers to study intracellular processes

Content Provider	IEEE Xplore Digital Library
Author	Faklaris, O. Garrot, D. Joshi, V. Druon, F. Boudou, J.P. Sauvage, T. Georges, P. Curmi, P. Treussart, F.
Copyright Year	2009
Description	Author affiliation: Laboratoire Charles Fabry de l'Institut d'Optique, UMR CNRS 8501, 2 av. Auguste Fresnel, 91127, Palaiseau, France (Druon, F.; Georges, P.) \|\| LMSSMat, Ecole Centrale Paris, UMR CNRS 8579, Grande Voie des Vignes, 92295 cedex, Chatenay-Malabry, France (Boudou, J.P.) \|\| Laboratoire de Photonique Quantique et Moléculaire, Ecole Normale Supérieure, UMR CNRS 8537, 94235 Cachan cedex, France (Faklaris, O.; Garrot, D.; Treussart, F.) \|\| Laboratoire Conditions Extrêmes et Matériaux: Haute Température et Irradiation, UPR CNRS 3079, Orléans, France (Sauvage, T.) \|\| Laboratoire Structure et Activité des Biomolécules Normales et Pathologiques, INSERM U829, Université d'Evry-Val d'Essonne, 91125, France (Joshi, V.; Curmi, P.)
Abstract	Single molecule observation is of great importance in life sciences. Individual probing enables studies of biomolecules interactions directly. In order to achieve an efficient biolabeling, a reliable marker is needed. Organic dyes and fluorophores are widely used, but they lack of photophysical stability. Alternative systems are Quantum dots (QDs). Although very efficient for multicolour staining and no photobleaching, they suffer of photoblinking and may be cytotoxic in long term. In contrast, diamond nanoparticles (NDs) containing nitrogen-vacancy (NV) color centers [1] represent a promising alternative to inorganic biomarkers. Indeed NV color centers neither photobleach nor blink and are biocompatible [2][3][4]. Here we demonstrate the efficient uptake of PNDs of size smaller than 50 nm at the single particle level in cultured cells. We improve cell imaging, examine for the first time thoroughly their internalization pathway, study the influence of their size, shape and surface groups to their internalization and compare their photoluminescence with that of QDs.
Starting Page	1
Ending Page	1
File Size	231407
Page Count	1
File Format	PDF
ISBN	9781424440795
DOI	10.1109/CLEOE-EQEC.2009.5191713
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2009-06-14
Publisher Place	Germany
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Nanoparticles Protons Stability Shape Microscopy Quantum dots Photoluminescence Cells (biology) Biomarkers Photobleaching
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Degradation related cytotoxicity of quantum dots

Second-harmonic efficiency of single barium titanate nanoparticles used as biomarkers for cells imaging

Comparison of the photoluminescence properties of semiconductor quantum dots and non-blinking diamond nanoparticles. Observation of the diffusion of diamond nanoparticles in living cells

Q-dots as probes for dynamic observation of intracellular pathways

Diamond nanoparticles as photoluminescent nanoprobes

Delivering quantum dots to cells: bioconjugated quantum dots for targeted and nonspecific extracellular and intracellular imaging

Diamond nanoparticles (904).

Structural and optical properties of columnar (In,Ga)As quantum dots on GaAs (100)

Analysis of photoluminescent properties of InAs/InGaAsP/InP quantum dots structure

Diamond photoluminescent nanoparticles as innovative markers to study intracellular processes

Similar Documents

Degradation related cytotoxicity of quantum dots

Second-harmonic efficiency of single barium titanate nanoparticles used as biomarkers for cells imaging

Comparison of the photoluminescence properties of semiconductor quantum dots and non-blinking diamond nanoparticles. Observation of the diffusion of diamond nanoparticles in living cells

Q-dots as probes for dynamic observation of intracellular pathways

Diamond nanoparticles as photoluminescent nanoprobes

Delivering quantum dots to cells: bioconjugated quantum dots for targeted and nonspecific extracellular and intracellular imaging

Diamond nanoparticles (904).

Structural and optical properties of columnar (In,Ga)As quantum dots on GaAs (100)

Analysis of photoluminescent properties of InAs/InGaAsP/InP quantum dots structure

Diamond photoluminescent nanoparticles as innovative markers to study intracellular processes