NDLI: Feedback control of a DNA molecule tethered in a nanopore to repeatedly probe DNA-binding enzymes

Content Provider	IEEE Xplore Digital Library
Author	Wilson, Noah A. Abu-Shumays, Robin Lieberman, Kate Akeson, Mark Dunbar, William B.
Copyright Year	2008
Description	Author affiliation: Baskin School of Engineering, University of California, Santa Cruz, 1156 High Street, CA, USA (Wilson, Noah A.; Abu-Shumays, Robin; Lieberman, Kate; Akeson, Mark; Dunbar, William B.)
Abstract	This paper demonstrates feedback voltage control of a single DNA molecule tethered in a biological nanopore. The nanopore device monitors ionic current through a single protein pore inserted in a lipid bilayer. The limiting aperture of the pore is just sufficient (1.5 nm diameter) to accommodate single-stranded DNA. The tethered DNA is double stranded on each end, with a single stranded segment that traverses the pore. Voltage control is used to regulate the motion of the tethered DNA, for repeated capture and subsequent voltage-promoted dissociation of DNA-binding enzymes above the nanopore. In initial experiments using the Klenow fragment of Escherichia coli DNA polymerase I, control of 8 independent tethered DNA molecules yielded 337 dissociation events in a period of 380 seconds. The resulting distribution of DNA-protein dissociation times can be used to model the free energy profile of dissociation. Moreover, the approach is applicable to numerous enzymes that bind or modify DNA or RNA including exonucleases, kinases, and other polymerases.
Starting Page	5745
Ending Page	5748
File Size	1797634
Page Count	4
File Format	PDF
ISBN	9781424418145
ISSN	1557170X
DOI	10.1109/IEMBS.2008.4650519
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-08-20
Publisher Place	Canada
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

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