NDLI: Experiment and mechanism research of SKOV3 cancer cell apoptosis induced by nanosecond pulsed electric field

Content Provider	IEEE Xplore Digital Library
Author	Chenguo Yao, Yan Mi, Xiaoqian Hu, Chengxiang Li, Caixin Sun, Junying Tang, Xiaojuan Wu,
Copyright Year	2008
Description	Author affiliation: State Key Laboratory of Power Transmission Equipment & System Security and New Technology, Chongqing University, 400044 China (Chenguo Yao,; Yan Mi,; Chengxiang Li,; Caixin Sun,) \|\| College of Electronic Information and Its Automation, Chongqing Institute of Technology, 400050 China (Xiaoqian Hu,) \|\| Chongqing University of Medical Science, 400010, China (Junying Tang,; Xiaojuan Wu,)
Abstract	This paper studies the apoptosis of human ovarian carcinoma cell Line (SKOV3) induced by the nanosecond pulsed electric field (10kV/cm, 100ns, 1 Hz) and its effect on intracellular calcium concentration $([Ca^{2+}]i).$ These cells were doubly marked by Annexin V-FITC/PI, and the apoptosis rate was analyzed with flow cytometry. After AO/EB staining the morphological changes were observed under fluorescent microscope, and their ultrastructural changes were observed under scanning electron microscope (SEM). With Fluo-3/AM as calcium fluorescent marker, laser scanning confocal microscope (LSCM) was used to detect the effect of nsPEF on $[Ca^{2+}]i$ and the source of $Ca^{2+}.$ The results showed that the early apoptosis rate of the treatment group was (22.21±2.71)%, significantly higher than that of the control group (3.04±0.44)% (P<0.01). The typical features of apoptotic cell have been observed by fluorescent microscope and SEM. It is proved that nsPEF can induce apoptosis of SKOV3 cells and result in distinct increase in $[Ca^{2+}]i$ (P<0.01), which was independent of extracellular calcium concentration (P>0.05). Since nsPEF can penetrate cell membrane due to its high frequency components, one of the mechanisms of nsPEF-induced apoptosis may be that activating intracellular calcium stores can increase the $[Ca^{2+}]i,$ and consequently, the apoptotic signal pathway can be induced.
Starting Page	1044
Ending Page	1047
File Size	521303
Page Count	4
File Format	PDF
ISBN	9781424418145
ISSN	1557170X
DOI	10.1109/IEMBS.2008.4649338
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-08-20
Publisher Place	Canada
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Ca²⁺ Nanosecond pulsed electric field (nsPEF) SKOV3 cancer cell apoptosis flow cytometry ultrastructure
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

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