| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Brenkman, Arjan B. Van Kooyk, Yvette Dijkstra, Christien D. Van Egmond, Marjolein Bruijns, Sven C. Bakema, Jantine E. Tuk, Cornelis W. Van Vliet, Sandra J. Vos, Joost B. Letsiou, Sophia |
| Description |
Author Affiliation: Bakema JE ( Department of Otolaryngology - Head and Neck Surgery, VU University Medical Center, 1007 MB Amsterdam, the Netherlands); Tuk CW ( Department of Molecular Cell Biology and Immunology, VU University Medical Center, 1081 BT Amsterdam, the Netherlands); van Vliet SJ ( Department of Molecular Cell Biology and Immunology, VU University Medical Center, 1081 BT Amsterdam, the Netherlands); Bruijns SC ( Department of Molecular Cell Biology and Immunology, VU University Medical Center, 1081 BT Amsterdam, the Netherlands); Vos JB ( Department of Molecular Cell Biology and Immunology, VU University Medical Center, 1081 BT Amsterdam, the Netherlands); Letsiou S ( Department of Metabolic Diseases, Netherlands Metabolomics Centre, University Medical Centre Utrecht, Utrecht 3584 EA, the Netherlands); Dijkstra CD ( Department of Molecular Cell Biology and Immunology, VU University Medical Center, 1081 BT Amsterdam, the Netherlands); van Kooyk Y ( Department of Molecular Cell Biology and Immunology, VU University Medical Center, 1081 BT Amsterdam, the Netherlands); Brenkman AB ( Department of Metabolic Diseases, Netherlands Metabolomics Centre, University Medical Centre Utrecht, Utrecht 3584 EA, the Netherlands); van Egmond M ( Department of Molecular Cell Biology and Immunology, VU University Medical Center, 1081 BT Amsterdam, the Netherlands) |
| Abstract |
During secondary immune responses, Ab-opsonized bacteria are efficiently taken up via FcRs by dendritic cells. We now demonstrate that this process induces cross-talk between FcRs and TLRs, which results in synergistic release of several inflammatory cytokines, as well as altered lipid metabolite profiles. This altered inflammatory profile redirects Th1 polarization toward Th17 cell responses. Interestingly, GM-CSF-producing Th cells were synergistically evoked as well, which suggests the onset of polyfunctional Th17 cells. Synergistic cytokine release was dependent on activation via MyD88 and ITAM signaling pathways through TLRs and FcRs, respectively. Cytokine regulation occurred via transcription-dependent mechanisms for TNF- and IL-23 and posttranscriptional mechanisms for caspase-1-dependent release of IL-1ß. Furthermore, cross-talk between TLRs and FcRs was not restricted to dendritic cells. In conclusion, our results support that bacteria alone initiate fundamentally different immune responses compared with Ab-opsonized bacteria through the combined action of two classes of receptors and, ultimately, may refine new therapies for inflammatory diseases. |
| ISSN |
00221767 |
| e-ISSN |
15506606 |
| Journal |
The Journal of Immunology |
| Issue Number |
4 |
| Volume Number |
194 |
| Language |
English |
| Publisher |
The American Association of Immunologists |
| Publisher Date |
2015-02-15 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
Dendritic Cells Immunology Receptor Cross-talk Receptors, Fc T-lymphocytes, Helper-inducer Toll-like Receptors Antibodies, Bacterial Blotting, Western Cell Differentiation Cell Separation Enterobacteriaceae Infections Enzyme-linked Immunosorbent Assay Escherichia Coli Flow Cytometry Immunologic Memory Inflammation Lymphocyte Activation Phenotype Real-time Polymerase Chain Reaction Signal Transduction T-lymphocyte Subsets Research Support, Non-u.s. Gov't Discipline Immunology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Immunology and Allergy Immunology |
