NDLI: Antigen-reactive T cell enrichment for direct, high-resolution analysis of the human naive and memory Th cell repertoire.

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Selective impairment of P2Y signaling by prostaglandin E2 in macrophages: implications for Ca2+-dependent responses.

Antigen-reactive T cell enrichment for direct, high-resolution analysis of the human naive and memory Th cell repertoire.

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Fibrin facilitates both innate and T cell-mediated defense against Yersinia pestis.

IL-1ß promotes antimicrobial immunity in macrophages by regulating TNFR signaling and caspase-3 activation.

Profibrotic activities for matrix metalloproteinase-8 during bleomycin-mediated lung injury.

Bcl6 expressing follicular helper CD4 T cells are fate committed early and have the capacity to form memory.

Functional genetic variation in NFKBIA and susceptibility to childhood asthma, bronchiolitis, and bronchopulmonary dysplasia.

Cutting edge: cell surface linker for activation of T cells is recruited to microclusters and is active in signaling.

Impact of immune complex size and glycosylation on IgG binding to human FcÎ³Rs.

Dose-dependent effects of IL-17 on IL-13-induced airway inflammatory responses and airway hyperresponsiveness.

Solute carrier 11A1 is expressed by innate lymphocytes and augments their activation.

The downregulation of microRNA let-7a contributes to the excessive expression of type I collagen in systemic and localized scleroderma.

Maintenance of anti-Sm/RNP autoantibody production by plasma cells residing in ectopic lymphoid tissue and bone marrow memory B cells.

Differential effects of phenethyl isothiocyanate and D,L-sulforaphane on TLR3 signaling.

1ß1 integrin-mediated adhesion inhibits macrophage exit from a peripheral inflammatory lesion.

A novel small-molecule enantiomeric analogue of traditional (-)-morphinans has specific TLR9 antagonist properties and reduces sterile inflammation-induced organ damage.

Coordinated changes in DNA methylation in antigen-specific memory CD4 T cells.

CD62L is critical for maturation and accumulation of murine hepatic NK cells in response to viral infection.

Altered T-bet dominance in IFN-Î³-decoupled CD4+ T cells with attenuated cytokine storm and preserved memory in influenza.

Anti-GM-CSF autoantibodies in patients with cryptococcal meningitis.

Inflammation induced by MMP-9 enhances tumor regression of experimental breast cancer.

Stepwise epigenetic and phenotypic alterations poise CD8+ T cells to mediate airway hyperresponsiveness and inflammation.

A new model for CD8+ T cell memory inflation based upon a recombinant adenoviral vector.

An extracellular matrix-based mechanism of rapid neutrophil extracellular trap formation in response to Candida albicans.

Monocyte- and macrophage-targeted NADPH oxidase mediates antifungal host defense and regulation of acute inflammation in mice.

PU.1-dependent transcriptional regulation of miR-142 contributes to its hematopoietic cell-specific expression and modulation of IL-6.

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Epithelial-mesenchymal transition induces an antitumor immune response mediated by NKG2D receptor.

Polyinosinic-polycytidylic acid is the most effective TLR adjuvant for SIV Gag protein-induced T cell responses in nonhuman primates.

Fas-mediated inflammatory response in Listeria monocytogenes infection.

Calcitonin gene-related peptide and cyclic adenosine 5'-monophosphate/protein kinase A pathway promote IL-9 production in Th9 differentiation process.

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Enhanced immunogenicity of a tricomponent mannan tetanus toxoid conjugate vaccine targeted to dendritic cells via Dectin-1 by incorporating ß-glucan.

Antisense oligonucleotide treatment enhances the recovery of acute lung injury through IL-10-secreting M2-like macrophage-induced expansion of CD4+ regulatory T cells.

TNFR-associated factor 6 regulates TCR signaling via interaction with and modification of LAT adapter.

Multiple transcription factor binding sites predict AID targeting in non-Ig genes.

Immunization with Escherichia coli outer membrane vesicles protects bacteria-induced lethality via Th1 and Th17 cell responses.

Complement in immune and inflammatory disorders: pathophysiological mechanisms.

Complement in immune and inflammatory disorders: therapeutic interventions.

Partial retraction. Antigen aggregation decides the fate of the allergic immune response.

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Antigen-reactive T cell enrichment for direct, high-resolution analysis of the human naive and memory Th cell repertoire.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kniemeyer, Olaf Bacher, Petra Teutschbein, Janka Schink, Christian Brakhage, Axel A. Assenmacher, Mario Scheffold, Alexander
Description	Country affiliation: Germany Author Affiliation: Bacher P ( Miltenyi Biotec, 51429 Bergisch Gladbach, Germany.)
Abstract	Ag-specific CD4(+) T cells orchestrating adaptive immune responses are crucial for the development of protective immunity, but also mediate immunopathologies. To date, technical limitations often prevented their direct analysis. In this study, we report a sensitive flow cytometric assay based on magnetic pre-enrichment of CD154(+) T cells to visualize rare Ag-reactive naive and memory Th cells directly from human peripheral blood. The detection limit of ≈ 1 cell within 10(5)-10(6) permitted the direct enumeration and characterization of auto-, tumor-, or neo-Ag-reactive T cells within the naive and even memory CD4(+) T cell repertoire of healthy donors. Furthermore, the analysis of high target cell numbers after pre-enrichment of rare Ag-specific T cells from large blood samples dramatically improved the identification of small subpopulations. As exemplified in this work, the dissection of the Ag-specific memory responses into small cytokine-producing subsets revealed great heterogeneity between pathogens, but also pathogen-related microsignatures refining Th cell subset classification. The possibility to directly analyze CD4(+) T cells reactive against basically any Ag of interest at high resolution within the naive and memory repertoire will open up new avenues to investigate CD4(+) T cell-mediated immune reactions and their use for clinical diagnostics.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	8
Volume Number	190
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2013-04-15
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cell Differentiation Immunology Immunologic Memory T-lymphocytes, Helper-inducer Metabolism Antigen Presentation Antigen-antibody Reactions Antigen-presenting Cells Aspergillus Fumigatus Cd4 Lymphocyte Count Cell Line Clone Cells Epitopes, T-lymphocyte T-lymphocyte Subsets Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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