| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Wang, Yi Wei, Xianzhao Xu, Jie Li, Quan Song, Shuang Lu, Zhe Chen, Qi Wang, Zimin |
| Description |
Author Affiliation: Wang Y ( Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.); Li Q ( Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.); Wei X ( Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.); Xu J ( Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.); Chen Q ( Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.); Song S ( Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.); Lu Z ( Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.); Wang Z ( Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.) |
| Abstract |
Subacromial bursitis (SAB) is the major source of pain in rotator cuff disease. Although multiple investigations have provided support for the role of inflammatory cytokines in SAB, few have focussed on the use these cytokines in the treatment of SAB. The aim of the present study was to observe the therapeutic efficacy of lentivirusmediated RNA interference (RNAi) on carrageenaninduced SAB by injecting lentivirustumor necrosis factor (TNF) RNAi expressing TNF small interfering (si)RNA. Using screened siRNA segments, an siRNA was designed. A lentivirus vector expressing siRNA was established and packed as lentivirus particles. A lentivirus that expressed the negative sequence was used as a lentivirusnegative control (NC). The carrageenaninduced SAB model was established in 32 male SpragueDawley rats. The modeled rats were randomly assigned to four groups: LentivirusRNAi treatment group, lentivirusNC group, SAB group and phosphatebuffered saline (PBS) blank control group. The lentivirus was injected (1x10(7) transducing units) into the subacromial bursa of the rats in the lentivirusRNAi group and lentivirusNC group, whereas 100 µl PBS was injected at the same site in the SAB group and the PBS blank control group. At 5 weeks following injection, the animals were sacrificed and venous blood was obtained. The effect of TNF interference and the expression of inflammatory cytokines were determined by reverse transcriptionquantitative polymerase chain reaction, western blotting, hematoxylin and eosin staining, Van Gieson's staining and immunofluorescence. The expression of TNF was decreased in the lentivirusTNF RNAi group compared with that in the SAB group. Morphological observations revealed that the number of inflammatory cells were reduced and damage to tendon fibers was attenuated in this group, suggesting that the downregulation of the protein expression levels of TNF associated nuclear factorκB, matrix metalloproteinase (MMP)1, MMP9, cyclooxygenase (COX)1 and COX2 may exert a therapeutic effect on inflammation of the SAB caused by rheumatoid arthritis. It was also found that the expression of stromal cellderived growth factor1 was downregulated in the lentivirusTNF RNAi group. Therefore, the present study demonstrated that lentivirusmediated TNF RNAi effectively inhibited the inflammatory response in SAB, and that injection of a lentivirus vector into the affected region is an effective way of achieving RNAi in vivo. |
| ISSN |
17912997 |
| e-ISSN |
17913004 |
| Journal |
Molecular Medicine Reports |
| Issue Number |
3 |
| Volume Number |
12 |
| Language |
English |
| Publisher |
Spandidos Publications |
| Publisher Date |
2015-09-01 |
| Publisher Place |
Greece |
| Access Restriction |
Open |
| Subject Keyword |
Bursitis Therapy Genetic Therapy Lentivirus Genetics Rna, Small Interfering Tumor Necrosis Factor-alpha Animals Blood Gene Expression Gene Knockdown Techniques Hek293 Cells Rna Interference Rats, Sprague-dawley Rotator Cuff Pathology Transduction, Genetic Biosynthesis Research Support, Non-u.s. Gov't Discipline Molecular Biology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Genetics Biochemistry Molecular Biology Cancer Research Molecular Medicine Oncology |
