| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Yang, Deyu Zhao, Mingjun Li, Dan Huang, Rongzhong Zhang, Liang Xie, Peng He, Peng Zhang, Hong Chen, Shigang Sun, Lin Liu, Xia Zhang, Lujun |
| Description |
Author Affiliation: Zhao M ( Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.); Sun L ( Institute of Neuroscience and the Collaborative Innovation Centre for Brain Science, Chongqing Medical University, Chongqing 400016, P.R. China.); Chen S ( Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.); Li D ( Institute of Neuroscience and the Collaborative Innovation Centre for Brain Science, Chongqing Medical University, Chongqing 400016, P.R. China.); Zhang L ( Institute of Neuroscience and the Collaborative Innovation Centre for Brain Science, Chongqing Medical University, Chongqing 400016, P.R. China.); He P ( Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.); Liu X ( Institute of Neuroscience and the Collaborative Innovation Centre for Brain Science, Chongqing Medical University, Chongqing 400016, P.R. China.); Zhang L ( Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.); Zhang H ( Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.); Yang D ( Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.); Huang R ( Institute of Neuroscience and the Collaborative Innovation Centre for Brain Science, Chongqing Medical University, Chongqing 400016, P.R. China.); Xie P ( Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.) |
| Abstract |
MicroRNAs (miRNAs) regulate gene expression by inhibiting transcription or translation and are involved in diverse biological processes, including development, cellular differentiation and tumor generation. miRNA microarray technology is a highthroughput global analysis tool for miRNA expression profiling. Here, the hippocampi of four borna disease virus (BDV)infected and four noninfected control neonatal rats were selected for miRNA microarray and bioinformatic analysis. Reverse transcription quantitative polymerase chain reaction (RTqPCR) analysis was subsequently performed to validate the dysregulated miRNAs. Seven miRNAs (miR145*, miR146a*, miR192*, miR200b, miR223*, miR449a and miR505), showed increased expression, whereas two miRNAs (miR126 and miR374) showed decreased expression in the BDVinfected group. By RTqPCR validation, five miRNAs (miR126, miR200b, miR374, miR449a and miR505) showed significantly decreased expression (P<0.05) in response to BDV infection. Biocarta pathway analysis predicted target genes associated with 'RNA', 'IGF1mTOR', 'EIF2', 'VEGF', 'EIF', 'NTHI', 'extrinsic', 'RB', 'IL1R' and 'IGF1' pathways. Gene Ontology analysis predicted target genes associated with 'peripheral nervous system development', 'regulation of small GTPase-mediated signal transduction', 'regulation of Ras protein signal transduction', 'aerobic respiration', 'membrane fusion', 'positive regulation of cell cycle', 'cellular respiration', 'heterocycle metabolic process', 'protein tetramerization' and 'regulation of Rho protein signal transduction' processes. Among the five dysregulated miRNAs identified by RTqPCR, miR126, miR200b and miR449a showed a strong association with nervous system development, cell differentiation, proliferation and apoptosis. |
| ISSN |
17912997 |
| e-ISSN |
17913004 |
| Journal |
Molecular Medicine Reports |
| Issue Number |
3 |
| Volume Number |
12 |
| Language |
English |
| Publisher |
Spandidos Publications |
| Publisher Date |
2015-09-01 |
| Publisher Place |
Greece |
| Access Restriction |
Open |
| Subject Keyword |
Animals, Newborn Virology Borna Disease Virus Genetics Bornaviridae Isolation & Purification Gene Expression Regulation, Developmental Hippocampus Micrornas Rats, Sprague-dawley Animals Growth & Development Apoptosis Cell Differentiation Cell Proliferation Gene Expression Profiling Metabolism Research Support, Non-u.s. Gov't Discipline Molecular Biology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Genetics Biochemistry Molecular Biology Cancer Research Molecular Medicine Oncology |
