| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Szebeni, Gábor J. Szabó, Emilia R. Hideghéty, Katalin Mán, Imola Hackler, László Szabó, Zoltán Nagy, Zoltán Plangár, Imola Fajka-Boja, Roberta Fekete, Gábor Puskás, László G. Tokés, Tünde |
| Description |
Country affiliation: Hungary Author Affiliation: Mán I ( Avidin Ltd., Szeged H6726, Hungary.); Szebeni GJ ( Avidin Ltd., Szeged H6726, Hungary.); Plangár I ( Department of Neurology, Faculty of Medicine, University of Szeged, Szeged H6725, Hungary.); Szabó ER ( Department of Oncotherapy, Faculty of Medicine, University of Szeged, Szeged H-6720, Hungary.); Tokés T ( Institute of Surgical Research, Faculty of Medicine, University of Szeged, Szeged H-6720, Hungary.); Szabó Z ( Department of Oncotherapy, Faculty of Medicine, University of Szeged, Szeged H-6720, Hungary.); Nagy Z ( Department of Oncotherapy, Faculty of Medicine, University of Szeged, Szeged H-6720, Hungary.); Fekete G ( Department of Oncotherapy, Faculty of Medicine, University of Szeged, Szeged H-6720, Hungary.); Fajka-Boja R ( Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged H-6726, Hungary.); Puskás LG ( Avidin Ltd., Szeged H6726, Hungary.); Hideghéty K ( Department of Oncotherapy, Faculty of Medicine, University of Szeged, Szeged H-6720, Hungary.); Hackler L ( Avidin Ltd., Szeged H6726, Hungary.) |
| Abstract |
Translational research in radiation oncology is important for the detection of adverse radiation effects, cellular responses, and radiation modifications, and may help to improve the outcome of radiation therapy in patients with cancer. The present study aimed to optimize and validate a realtime labelfree assay for the dynamic monitoring of cellular responses to ionizing radiation. The xCELLigence system is an impedancebased platform that provides continuous information on alterations in cell size, shape, adhesion, proliferation, and survival. In the present study, various malignant human primary fibroblast cells (U251, GBM2, MCF7, A549, HT29) were exposed to 0, 5 and 10 Gy of Cobalt60 radiation. As well as the xCELLigence system, cell survival and proliferation was evaluated using the following conventional endpoint cellbased methods: Clonogenic, MTS, and lactate dehydrogenase assays, and apoptosis was detected by fluorescenceactivated cell sorting. The effects of ionizing radiation were detected for each cell line using impedance monitoring. The realtime data correlated with the colony forming assay results. At low cell densities (1,0002,000 cells/well) the impedancebased method was more accurate at monitoring dosedependent changes in the malignant human primary fibroblast cell lines, as compared with the endpoint assays. The results of the present study demonstrated that the xCELLigence system may be a reliable and rapid diagnostic method for the monitoring of dynamic cell behavior following radiation. In addition, the xCELLigence system may be used to investigate the cellular mechanisms underlying the radiation response, as well as the timedependent effects of radiation on cell proliferation and viability. |
| ISSN |
17912997 |
| e-ISSN |
17913004 |
| Journal |
Molecular Medicine Reports |
| Issue Number |
3 |
| Volume Number |
12 |
| Language |
English |
| Publisher |
Spandidos Publications |
| Publisher Date |
2015-09-01 |
| Publisher Place |
Greece |
| Access Restriction |
Open |
| Subject Keyword |
Apoptosis Radiation Effects Biological Assay Instrumentation Fibroblasts Gamma Rays Cell Adhesion Cell Count Cell Line, Tumor Cell Proliferation Cell Shape Cell Size Cell Survival Dose-response Relationship, Radiation Electric Impedance Cytology Enzymology L-lactate Dehydrogenase Metabolism Organ Specificity Primary Cell Culture Time Factors Research Support, Non-u.s. Gov't Discipline Molecular Biology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Genetics Biochemistry Molecular Biology Cancer Research Molecular Medicine Oncology |
