| Author |
Li, Xiaolan Hu, Junbo Luo, Xuelai Leng, Yan Gong, Jianping Hu, Fayong Yang, Rui Tao, Deding Liu, Anding Jiang, Jianwu Xu, Xiangshang |
| Description |
Author Affiliation: Li X ( Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.); Jiang J ( Department of Gastrointestinal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.); Yang R ( Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.); Xu X ( Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.); Hu F ( Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.); Liu A ( Experimental Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.); Tao D ( Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.); Leng Y ( Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.); Hu J ( Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.); Gong J ( Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.); Luo X ( Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China.) |
| Abstract |
Colorectal cancer (CRC) is one of the most common types of cancer worldwide. Hematopoietic cellspecific protein 1associated protein X1 (HAX1) has been found to be involved in several types of cancer. However, the role of HAX1 in CRC remains to be elucidated. The aim of the present study was to investigate whether the expression of HAX1 is associated with the progression of CRC, and to determine the effects of HAX1 on the apoptosis and proliferation of CRC cells. Tumor tissues and adjacent noncancerous tissues were collected from 60 patients with CRC, following the provision of informed consent. The expression levels of HAX1 and the association with clinical and pathological characteristics were then analyzed. The expression levels of HAX1 were significantly higher in the cancerous tissues from the patients with CRC, particularly in tissues of an advanced stage of cancer. In addition, HAX1 expression was associated with malignant progression and poor prognosis. Furthermore, SW480 CRC cells, overexpressing HAX1, exhibited increased resistance to camptothecin in vitro, and promoted proliferation in vitro and in vivo. By contrast, HAX1 knockdown significantly decreased the proliferation. In addition, the expression levels of ki67 and phosphorylatedakt were inhibited following HAX1 knockdown. In conclusion, the expression levels of HAX1 were increased in cancerous tissue from patients with CRC, and were associated with progression of the disease. These results suggested that HAX1 may contribute to chemotherapy resistance and malignant progression in CRC. |
