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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Han, Huan-Wen Chang, Hsien-Chang Cheng, I-Fang |
| Description | Author Affiliation: Cheng IF ( Department of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan, ROC.) |
| Abstract | We present a dielectrophoresis (DEP)-based microfluidic chip that is capable of enhancing the sensitivity and selectivity of DNA hybridization using an AC electric field and hydrodynamic shear in a continuous through-flow. Molecular DEP was employed to rapidly trap ssDNA molecules in a flowing solution to a cusp-shaped nanocolloid assembly on a microfluidic chip with a locally amplified AC electric field gradient. The detection time can be accelerated to sub-minute periods, and the sensitivity can reach the pico-molar level due to the AC DEP-enhanced molecule concentration (at an optimal AC frequency of 900 kHz) in a small region (â ¼100 µm(2)) instead of the broad area used in a tank reactor (â ¼10(6) µm(2)). Continuous flow in a microchannel provides a constant and high shear rate that can shear off most non-specific target-probe binding to promote the discriminating selectivity. On-chip multi-target discrimination of Candida species can be achieved within a few minutes under optimal conditions. |
| ISSN | 09565663 |
| Issue Number | 1 |
| Volume Number | 33 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-03-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Candida Isolation & Purification Dna Genetics Microfluidic Analytical Techniques Methods Nucleic Acid Hybridization Polymerase Chain Reaction Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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