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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Spadafora, Lauren J. Trau, Matt Shiddiky, Muhammad J. A. Cangelosi, Gerard A. Grewal, Yadveer S. |
| Description | Country affiliation: Australia Author Affiliation: Grewal YS ( Centre for Biomarker Research and Development, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane QLD 4072, Australia.); Shiddiky MJ ( Centre for Biomarker Research and Development, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane QLD 4072, Australia. Electronic address: m.shiddiky@uq.edu.au.); Spadafora LJ ( School of Public Health University of Washington, Seattle, WA, USA.); Cangelosi GA ( School of Public Health University of Washington, Seattle, WA, USA.); Trau M ( Centre for Biomarker Research and Development, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane QLD 4072, Australia) |
| Abstract | The time and costs associated with monoclonal antibody production limit the potential for portable diagnostic devices to penetrate the market. Replacing the antibody with a low-cost alternate affinity reagent would reduce the costs of diagnostic development and use, and lead to new portable diagnostic devices towards many diseases. Herein, we present low-cost affinity reagents, nano-yeast-scFv, on commercially available, inexpensive, and portable screen-printed electrodes for the label-free electrochemical detection of Entamoeba histolytica cyst antigens. The biosensor was able to detect antigen at concentrations down to 10 pg mL(-1) in buffer with an inter-assay reproducibility of (% RSD, n=3) 4.1%. The applicability of two differently engineered nano-yeast-scFv to each specifically detect their cognant E. histolytica cyst antigens was demonstrated in a biological matrix derived from human stool. Because of the simple, inexpensive, and sensitive nature of this methodology, it may offer a low-cost alternative to immunosensors based on antibody-target recognition. |
| ISSN | 09565663 |
| Volume Number | 55 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-05-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Antigens, Protozoan Analysis Conductometry Instrumentation Electrodes Entamoeba Histolytica Immunology Feces Parasitology Immunoassay Single-chain Antibodies Biosensing Techniques Isolation & Purification Equipment Design Equipment Failure Analysis Fungal Proteins Gold Metal Nanoparticles Chemistry Molecular Probe Techniques Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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