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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Hu, Kun Zhao, Yanchun Chen, Sheng He, Yanlong Tian, Jianniao Zhang, Juanni Zhao, Shulin Jiang, Yixuan |
| Description | Country affiliation: China Author Affiliation: He Y ( Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China.); Tian J ( Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China. Electronic address: birdtjn@sina.com.); Zhang J ( Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China.); Chen S ( Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China.); Jiang Y ( Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China.); Hu K ( Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China.); Zhao Y ( Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China. Electronic address: yanchunzh@sina.com.); Zhao S ( Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China.) |
| Abstract | In the paper, we have constructed a very simple, sensitive and promising assay for fluorescence biosensor detection of Cu(2+) in aqueous solutions based on FRET between gold nanorods (AuNRs) and the 3'-TAMRA-labeled substrate strand (Sub) of DNAzyme. The fluorescence of the Sub is quenched when the substrate strand-DNAzyme strand (Sub-Enz) duplex is adsorbed on AuNRs surface through electrostatic interaction. In the presence of Cu(2+), the fluorescence is restored due to the decrease of FRET efficiency caused by the specific cleavage of the Sub by the DNAzyme (Enz), which weakens the electrostatic interaction between the AuNRs and short 3'-TAMRA-labeled DNA fragment. This method shows a high sensitivity for Cu(2+) with a detection limit of 9.83 pM (S/N=3) and a linear range from 0.016 nM to 40 nM. At the same time, Cu(2+) can be detected sensitively based on the significantly decreased Fluorescent polarization (FP). The detection limit of 8.40 pM is experimentally achieved for Cu(2+). |
| ISSN | 09565663 |
| Volume Number | 55 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-05-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Copper Analysis Dna, Catalytic Chemistry Fluorescence Resonance Energy Transfer Gold Nanotubes Refractometry Enzymes, Immobilized Equipment Design Equipment Failure Analysis Ions Metal Nanoparticles Ultrastructure Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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