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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ji, Kaili Chan, Mark Yau Li, Sam Fong Seneviratnankn, Aruni Bi, Xuezhi de Carvalho, Leonardo Pinto Bhakoo, Kishore |
| Description | Country affiliation: Singapore Author Affiliation: Ji K ( NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore 119077, Singapore.); de Carvalho LP ( Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.); Bi X ( Bioprocessing Technology Institute, Agency for Science, Technology and Research, 20 Biopolis Way, #06-01, Singapore 138668, Singapore.); Seneviratnankn A ( Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.); Bhakoo K ( Translational Molecular Imaging Group, Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore 138667, Singapore.); Chan M ( National University Heart Centre, Singapore 119074, Singapore.); Yau Li SF ( Department of Chemistry and NUS Environmental Research Institute, National University of Singapore, Singapore 117543, Singapore) |
| Abstract | Aptamer-based biosensors (aptasensor) are powerful tools for rapid and sensitive biomarker detection. In this study, we report a DNA aptamer probe evolved from cell-SELEX that can recognize thrombospondin-1 protein in human plasma samples. The KD value of the aptamer M55 binding to thrombospondin-1 was determined as 0.5 ± 0.2 µM with an R(2) of 0.9144. A horseradish peroxidase-linked short oligo was complementarily bound onto the 3' end of the aptamer sequence to facilitate the 'smart' design of an M55-aptasensor for quantifying thrombospondin-1 protein in plasma samples. The limit of detection was 6.96 fM. Thrombospondin-1 is a glycoprotein with multiple biological functions, including inflammation, platelet aggregation and endothelial cell apoptosis, and is involved in the pathology of atherosclerosis. In total, 118 plasma subjects were analyzed by using the aptasensor measurement with 1 µL sample volume and 5 min incubation time. The thrombospondin-1 concentrations in ST-Elevation Myocardial Infarction patients with severe atherosclerotic plaque burden were statistically significantly higher than in the healthy volunteers without atherosclerosis conditions, suggesting that thromboposnidn-1 is a potential plasma biomarker for atherosclerosis progression. |
| ISSN | 09565663 |
| Volume Number | 55 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-05-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Aptamers, Nucleotide Genetics Biosensing Techniques Instrumentation Coronary Artery Disease Blood Diagnosis Dna Probes Thrombospondin 1 Chemistry Equipment Design Equipment Failure Analysis Humans Reproducibility Of Results Sensitivity And Specificity Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Validation Studies Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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