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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Eguílaz, Marcos Yáñez-Sedeño, Paloma Pingarrón, José M. Campuzano, Susana González-Cortés, Araceli Moreno-Guzmán, María |
| Description | Country affiliation: Spain Author Affiliation: Eguílaz M ( Department of Analytical Chemistry, Faculty of Chemistry, University Computense of Madrid, Ciudad Universitaria, 28040 Madrid, Spain.) |
| Abstract | A disposable electrochemical immunosensor using screen-printed carbon electrodes (SPCEs) and protein A-functionalized magnetic beads (MBs) was developed for the determination of testosterone. Anti-testosterone was immobilized onto MBs and a direct competitive immunoassay involving testosterone labeled with peroxidase (HRP) was performed. The resulting conjugate was trapped on the SPCE with a small magnet. Testosterone determination was carried out by amperometry at -0.2V upon H2O2 additions using hydroquinone (HQ) as the redox mediator. The experimental variables involved in the immunosensor response to testosterone were evaluated. Under the optimized conditions, a calibration plot for testosterone was obtained with a linear range between 5.0×10(-3) and 50 ng/mL (r=0.995). The detection limit was 1.7 pg/mL and the EC50 was 0.25±0.04 ng/mL. These characteristics are notably better than those achieved with other reported immunosensors. Furthermore, anti-testosterone/MBs conjugates were shown to be stable for at least 25 days. A good selectivity was also found against other steroid hormones. The usefulness of the immunosensor was demonstrated by analyzing human serum spiked with 1 and 10 ng/mL testosterone. |
| ISSN | 09565663 |
| Issue Number | 2 |
| Volume Number | 26 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-10-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Carbon Chemistry Conductometry Electrodes Immunomagnetic Separation Testosterone Blood Disposable Equipment Equipment Design Equipment Failure Analysis Immunology Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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