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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Yadav, Saurabh K. Goyal, Rajendra N. Chandra, Pranjal Agrawal, Bharati |
| Description | Country affiliation: India Author Affiliation: Yadav SK ( Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India.); Agrawal B ( Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India.); Chandra P ( Amity Institute of Biotechnology, Amity University Uttar Pradesh, Gautam Buddha Nagar, Noida 201303, Uttar Pradesh, India.); Goyal RN ( Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India. Electronic address: rngcyfcy@iitr.ac.in.) |
| Abstract | A sensitive and selective electrochemical biosensor is developed for the determination of chloramphenicol (CAP) exploring its direct electron transfer processes in in-vitro model and pharmaceutical samples. This biosensor exploits a selective binding of CAP with aptamer, immobilized onto the poly-(4-amino-3-hydroxynapthalene sulfonic acid) (p-AHNSA) modified edge plane pyrolytic graphite. The electrochemical reduction of CAP was observed in a well-defined peak. A quartz crystal microbalance (QCM) study is performed to confirm the interaction between the polymer film and the aptamer. Cyclic voltammetry (CV) and square wave voltammetry (SWV) were used to detect CAP. The in-vitro CAP detection is performed using the bacterial strain of Haemophilus influenza. A significant accumulation of CAP by the drug sensitive H. influenza strain is observed for the first time in this study using a biosensor. Various parameters affecting the CAP detection in standard solution and in in vitro detection are optimized. The detection of CAP is linear in the range of 0.1-2500 nM with the detection limit and sensitivity of 0.02 nM and 0.102 µA/nM, respectively. CAP is also detected in the presence of other common antibiotics and proteins present in the real sample matrix, and negligible interference is observed. |
| ISSN | 09565663 |
| Volume Number | 55 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-05-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Aptamers, Nucleotide Genetics Chloramphenicol Analysis Pharmacology Conductometry Instrumentation Haemophilus Influenzae Drug Effects Micro-electrical-mechanical Systems Polymers Chemistry Anti-bacterial Agents Biological Assay Biosensing Techniques Dose-response Relationship, Drug Equipment Design Equipment Failure Analysis Isolation & Purification Physiology Immunoassay Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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