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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Dharuman, Venkataraman Shankara Narayanan, Jeyaraman Bhuvana, Mohanlal |
| Description | Country affiliation: India Author Affiliation: Shankara Narayanan J ( Molecular Electronics Laboratory, Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi 630003, India.); Bhuvana M ( Molecular Electronics Laboratory, Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi 630003, India.); Dharuman V ( Molecular Electronics Laboratory, Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi 630003, India. Electronic address: dharumanudhay@yahoo.com.) |
| Abstract | Cationic N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium propane (DOTAP) liposome is spherically sandwiched in gold nanoparticle (abbreviated as sDOTAP-AuNP) onto a gold electrode surface. The sDOTAP-AuNP is applied for electrochemical label free DNA sensing and Escherichia coli cell transfection for the first time. Complementary target (named as hybridized), non-complementary target (un-hybridized) and single base mismatch target (named as SMM) hybridized surfaces are discriminated sensitively and selectively in presence of [Fe(CN)6](3-/4-). Double strand specific intercalator methylene blue in combination with [Fe(CN)6](3-) is used to enhance target detection limit down to femtomolar concentration. Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), differential pulse voltammetry (DPV) techniques are used for characterizing DNA sensing. High Resolution Transmission Electron Microscopy (HRTEM), Fourier Transform Infrared Spectroscopy (FTIR), Atomic Force Microscopy (AFM) and Dynamic Light Scattering (DLS) techniques are used to confirm the spherical nature of the sDOTAP-AuNP-DNA composite in solution and on the solid surface. DNA on the sDOTAP-ssDNA is transferred by potential stripping method (+0.2V (Ag/AgCl)) into buffer solution containing E. coli cells. The transfection is confirmed by the contrast images for the transfected and non-transfected cell from Confocal Laser Scanning Microscopy (CLSM). The results demonstrate effectiveness of the electrochemical DNA transfection method developed and could be applied for other cells. |
| ISSN | 09565663 |
| Volume Number | 58 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-08-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Conductometry Instrumentation Dna, Bacterial Genetics Escherichia Coli Gold Chemistry Metal Nanoparticles Sequence Analysis, Dna Transfection Biosensing Techniques Analysis Equipment Design Equipment Failure Analysis Liposomes Ultrastructure Microelectrodes Transducers Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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