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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhou, Bin Wang, Yong-Song Yin, Ji-Cheng Qian, Qiu-Mei Zhao, Hui Liu, Hui Liu, Shan-Du Xue, Jin-Hua Wang, Yong-Sheng Wang, Jia-Cheng |
| Description | Author Affiliation: Wang JC ( College of Public Health, University of South China, Hengyang 421001, PR China); Wang YS ( College of Public Health, University of South China, Hengyang 421001, PR China. Electronic address: yongsheng.w@tom.com.); Xue JH ( College of Public Health, University of South China, Hengyang 421001, PR China.); Zhou B ( College of Public Health, University of South China, Hengyang 421001, PR China.); Qian QM ( College of Public Health, University of South China, Hengyang 421001, PR China.); Wang YS ( College of Public Health, University of South China, Hengyang 421001, PR China.); Yin JC ( College of Public Health, University of South China, Hengyang 421001, PR China.); Zhao H ( College of Public Health, University of South China, Hengyang 421001, PR China.); Liu H ( College of Public Health, University of South China, Hengyang 421001, PR China.); Liu SD ( College of Public Health, University of South China, Hengyang 421001, PR China.) |
| Abstract | We developed a highly sensitive label-free assay of 8-hydroxy-2'-deoxyguanosine (8-OHdG) using 8-OHdG-aptamer (Apt) as the recognition element. The Apt was adsorbed onto the surface of gold nanoparticles (AuNPs), which prevents them from aggregating under high-salt conditions. Upon addition of 8-OHdG, the conformation of the Apt changes to form a G-quadruplex structure, which leads to the aggregation of the AuNPs along with the increase of the resonance light scattering intensity. The mechanism of 8-OHdG that induces Apt to form G-quadruplexes structure was studied by circular dichroism. The response signals linearly correlated with the concentration of 8-OHdG ranging from 90.8pM to 14.1nM with a detection limit of 27.3pM, which is much lower than that obtained by other methods. This method does not need any label steps and sophisticated equipment. The application for detection of 8-OHdG in real samples further demonstrated its reliability. This strategy would be helpful for developing a universal analytical method by simply replacing corresponding aptamers for various target molecules. |
| ISSN | 09565663 |
| Volume Number | 58 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-08-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Aptamers, Nucleotide Chemistry Biosensing Techniques Instrumentation Circular Dichroism Methods Deoxyguanosine Analogs & Derivatives Gold Metal Nanoparticles Genetics Analysis Equipment Design Equipment Failure Analysis Ultrastructure Microchemistry Nucleic Acid Conformation Staining And Labeling Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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