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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Swainsbury, David J. K. Jones, Michael R. Frese, Raoul N. Friebe, Vincent M. |
| Description | Author Affiliation: Swainsbury DJ ( School of Biochemistry, Medical Sciences Building, University of Bristol, University Walk, Bristol BS8 1TD, United Kingdom. Electronic address: D.Swainsbury@bristol.ac.uk.); Friebe VM ( Division of Physics and Astronomy, Department of Biophysics, VU University Amsterdam, De Boelelaan 1081, Amsterdam 1081 HV, The Netherlands. Electronic address: v.m.friebe@vu.nl.); Frese RN ( Division of Physics and Astronomy, Department of Biophysics, VU University Amsterdam, De Boelelaan 1081, Amsterdam 1081 HV, The Netherlands. Electronic address: r.n.frese@vu.nl.); Jones MR ( School of Biochemistry, Medical Sciences Building, University of Bristol, University Walk, Bristol BS8 1TD, United Kingdom. Electronic address: m.r.jones@bristol.ac.uk.) |
| Abstract | The Rhodobacter sphaeroides reaction centre is a relatively robust and tractable membrane protein that has potential for exploitation in technological applications, including biohybrid devices for photovoltaics and biosensing. This report assessed the usefulness of the photocurrent generated by this reaction centre adhered to a small working electrode as the basis for a biosensor for classes of herbicides used extensively for the control of weeds in major agricultural crops. Photocurrent generation was inhibited in a concentration-dependent manner by the triazides atrazine and terbutryn, but not by nitrile or phenylurea herbicides. Measurements of the effects of these herbicides on the kinetics of charge recombination in photo-oxidised reaction centres in solution showed the same selectivity of response. Titrations of reaction centre photocurrents yielded half maximal inhibitory concentrations of 208nM and 2.1µM for terbutryn and atrazine, respectively, with limits of detection estimated at around 8nM and 50nM, respectively. Photocurrent attenuation provided a direct measure of herbicide concentration, with no need for model-dependent kinetic analysis of the signal used for detection or the use of prohibitively complex instrumentation, and prospects for the use of protein engineering to develop the sensitivity and selectivity of herbicide binding by the Rba. sphaeroides reaction centre are discussed. |
| ISSN | 09565663 |
| e-ISSN | 18734235 |
| DOI | 10.1016/j.bios.2014.02.050 |
| Journal | Biosensors and Bioelectronics |
| Volume Number | 58 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-08-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | Open |
| Subject Keyword | Biosensing Techniques Instrumentation Electrochemistry Herbicides Photochemistry Photosynthetic Reaction Center Complex Proteins Chemistry Rhodobacter Sphaeroides Triazines Equipment Design Equipment Failure Analysis Radiation Effects Evaluation Studies Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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