NDLI: Terahertz absorption of illuminated photosynthetic reaction center solution: a signature of photoactivation?

Content Provider	Royal Society of Chemistry (RSC)
Author	Wahlgren, Weixiao Y. Pietro, Paola Di Katona, Gergely Lupi, Stefano Lundholm, Ida Duelli, Annette Piccirilli, Federica Perucchi, Andrea Berntsson, Oskar
Copyright Year	2014
Abstract	Photosynthetic reaction centers develop a stable charge separated state upon illumination. To investigate the molecular vibrations associated with the illuminated state of a reaction center we recorded terahertz absorption spectra of the photosynthetic reaction center from Rhodobacter sphaeroides in the dark and upon illumination and observed a small, but significant THz absorption increase in the 20 to 130 cm−1 spectral region. Reaction centers show very similar terahertz absorption increase when solubilized in detergents and in a lipidic sponge phase indicating that the nature of the bulk solvent has limited influence on the vibrational spectrum. The absorption change of the isolated LM subunit is very similar to that of the intact reaction center. Through temperature control experiments we show that 89% of the absorption change is likely attributed to the non-thermal activation of the protein molecules. These results indicate that picosecond molecular vibrations change primarily in the cofactors and/or in the evolutionary conserved core of the reaction center upon illumination, whereas the nuclear motions of the H-subunit and the bulk solvent have limited impact on the terahertz spectral changes.
Starting Page	25502
Ending Page	25509
Page Count	8
File Format	HTM / HTML PDF
ISSN	20462069
Volume Number	4
Issue Number	49
Journal	RSC Advances
DOI	10.1039/c4ra03787a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Photosynthetic reaction centre Picosecond Rhodobacter sphaeroides Terahertz radiation Cofactor (biochemistry) Solvent Coordination complex Protein
Content Type	Text
Resource Type	Article
Subject	Chemistry Chemical Engineering

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