NDLI: DNA-based biosensors for Hg(2+) determination by polythymine-methylene blue modified electrodes.

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DNA-based biosensors for Hg(2+) determination by polythymine-methylene blue modified electrodes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bollella, Paolo Antiochia, Riccarda Tortolini, Cristina Antonelli, Marta Letizia Favero, Gabriele Mazzei, Franco
Description	Country affiliation: Italy Author Affiliation: Tortolini C ( Department of Chemistry and Drug Technologies, Sapienza University of Rome, Italy); Bollella P ( Department of Chemistry, Sapienza University of Rome, Italy.); Antonelli ML ( Department of Chemistry, Sapienza University of Rome, Italy.); Antiochia R ( Department of Chemistry and Drug Technologies, Sapienza University of Rome, Italy.); Mazzei F ( Department of Chemistry and Drug Technologies, Sapienza University of Rome, Italy.); Favero G ( Department of Chemistry and Drug Technologies, Sapienza University of Rome, Italy.)
Abstract	In this work we have developed a new electrochemical DNA-based biosensor for the selective determination of the Hg(2+) ion by the use of different electrodes modified with polythymine, bearing methylene blue, as redox probe, in 3' position. The determination of Hg(2+) can be employed with an excellent degree of selectivity by the use of DNA biosensors through the formation of the complex Thymine-Hg-Thymine (T-Hg-T): in fact, Hg(2+) tends to bind two thymines, generating a T-Hg-T complex with a formation constant higher than that one of the coupling Adenine-Thymine, which can be employed for a selective, fast and cost-effective Hg(2+) detection. The presence of the Hg(2+) in solution leads to the formation of T-Hg-T complex thus causing the 'hairpin-like' folding of oligonucleotide, leading to an improved electronic exchange of methylene blue with the electrode surface due to the reduced distance and thus to an increase of the faradic current which is detected by means of square wave voltammetry (SWV). To test the feasibility of this kind of biosensor to be applied to the analysis of Hg(2+) we have developed several biosensors configuration by modifying the electrochemical sensor transducer: (a) Au electrode; (b) Au screen-printed electrode (SPE). The proposed system, allows the determination of Hg(2+) in the range 0.2-100 nM (0.05-20 ppb), with a sensitivity 0.327 µA/nM, LOD 0.1 nM (0.02 ppb), LOQ 0.2 nM (0.05 ppb) and RSD ≤4.3% when Au electrode is used as electrochemical transducer; on the other hand, in the case of Au SPE the linear range is 0.2-50 nM (0.05-10 ppb), with a sensitivity 0.285 µA/nM, while LOD and LOQ are the same as previously and RSD is ≤3.8%. This enabled the detection of mercury in real samples (waters and fishes) with good accuracy (recoveries 92-101% on waters and 92-107% on fishes, respectively) and reproducibility (RSD ≤9.6% for measurements on waters and ≤8.8% on fishes, respectively).
ISSN	09565663
Volume Number	67
e-ISSN	18734235
Journal	Biosensors and Bioelectronics
Language	English
Publisher	Elsevier
Publisher Date	2015-05-15
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biosensing Techniques Dna Chemistry Mercury Isolation & Purification Animals Fishes Blood Gold Limit Of Detection Water Analysis Journal Article Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry

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