NDLI: Integrated potentiostat for electrochemical sensing of urinary 3-hydroxyanthranilic acid with molecularly imprinted poly(ethylene-co-vinyl alcohol).

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Integrated potentiostat for electrochemical sensing of urinary 3-hydroxyanthranilic acid with molecularly imprinted poly(ethylene-co-vinyl alcohol).

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chao, I-Jen Lin, Hung-Yin Lee, Mei-Hwa O'Hare, Danny Huang, Chun-Yueh Liang, Yi-Fan Wei, Hung-Wei Liu, Bin-Da
Description	Country affiliation: Taiwan Author Affiliation: Huang CY ( Department of Electrical Engineering, National University of Tainan, Tainan, Taiwan.); O'Hare D ( Department of Bioengineering, Imperial College, London SW7 2BY, United Kingdom.); Chao IJ ( Department of Electrical Engineering, National Cheng Kung University, Tainan, Taiwan.); Wei HW ( Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan.); Liang YF ( Department of Electrical Engineering, National University of Tainan, Tainan, Taiwan.); Liu BD ( Department of Electrical Engineering, National Cheng Kung University, Tainan, Taiwan.); Lee MH ( Department of Materials Science and Engineering, I-Shou University, Kaohsiung 840, Taiwan.); Lin HY ( Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan. Electronic address: linhy@ntu.edu.tw.)
Abstract	Changing demographics, the rise of personalized medicine and increased identification of biomarkers for diagnosis and management of chronic disease have increased the demand for portable bioanalytical instrumentation and point-of-care. The recent development of molecularly imprinted polymers enables production of low cost and highly stable sensing chips; however, the commercially available and full functional instruments employed for electrochemical analysis have shortcomings in actual homecare applications. In this work, integrated circuits (ICs) for monolithic implementation of voltammeter potentiostat with a large dynamic current range (5 nA to 1.2 mA) and short conversion time (10 ms) were fabricated in a 0.35 µm complementary metal-oxide-semiconductor (CMOS) process. The new instrumentation was tested with molecular imprinted sensors for 3-hydroxyanthranilic acid (3HAA) in urine. The sensor consisted of molecular imprinted of poly(ethylene-co-vinyl alcohol)s (abbreviated as EVALs) for implementation in a flow injection analysis system. The EVAL containing 32 ethylene mol% had the highest imprinting effectiveness for the target molecules. Fit-for-purpose figures of merit were achieved with a limit-of-detection (LOD) of 3.06 pg/mL. The measurements obtained in real undiluted urine samples fell within the reference concentration range of 50-550 ng/mL.
ISSN	09565663
Volume Number	67
e-ISSN	18734235
Journal	Biosensors and Bioelectronics
Language	English
Publisher	Elsevier
Publisher Date	2015-05-15
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	3-hydroxyanthranilic Acid Analysis Conductometry Instrumentation Molecular Imprinting Methods Polyvinyls Chemistry Potentiometry Urinalysis Equipment Design Equipment Failure Analysis Reproducibility Of Results Sensitivity And Specificity Systems Integration Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry

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