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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Liu, Yu-Jie Huang, Ke-Jing Cao, Jun-Tao Liu, Yan-Ming Zhang, Ji-Zong |
| Description | Author Affiliation: Huang KJ ( College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China); Liu YJ ( College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.); Zhang JZ ( College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.); Cao JT ( College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.); Liu YM ( College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.) |
| Abstract | We have developed a sensitive sensing platform for 17ß-estradiol by combining the aptamer probe and hybridization reaction. In this assay, 2-dimensional cobalt sulfide nanosheet (CoS) was synthesized by a simple hydrothermal method with L-cysteine as sulfur donor. An electrochemical aptamer biosensor was constructed by assembling a thiol group tagged 17ß-estradiol aptamer on CoS and gold nanoparticles (AuNPs) modified electrode. Methylene blue was applied as a tracer and a guanine-rich complementary DNA sequence was designed to bind with the unbound 17ß-estradiol aptamer for signal amplification. The binding of guanine-rich DNA to the aptamer was inhibited when the aptamer captured 17ß-estradiol. Using guanine-rich DNA in the assay greatly amplified the redox signal of methylene blue bound to the detection probe. The CoS/AuNPs film formed on the biosensor surface appeared to be a good conductor for accelerating the electron transfer. The method demonstrated a high sensitivity of detection with the dynamic concentration range spanning from 1.0×10(-9) to 1.0×10(-12) M and a detection limit of 7.0×10(-13) M. Besides, the fabricated biosensor exhibited good selectivity toward 17ß-estradiol even when interferents were presented at 100-fold concentrations. Our attempt will extend the application of the CoS nanosheet and this signal amplification assay to biosensing areas. |
| ISSN | 09565663 |
| Volume Number | 67 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-05-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Aptamers, Nucleotide Chemistry Biosensing Techniques Instrumentation Conductometry Dna, Complementary Estradiol Analysis Metal Nanoparticles Genetics Cobalt Equipment Design Equipment Failure Analysis Gold Guanine Ultrastructure Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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