NDLI: Simple and rapid CD4 testing based on large-field imaging system composed of microcavity array and two-dimensional photosensor.

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Preface. Biosensors 2014.

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Simple and rapid CD4 testing based on large-field imaging system composed of microcavity array and two-dimensional photosensor.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lim, Tae-Kyu Harada, Manabu Hosokawa, Masahito Saeki, Tatsuya Matsunaga, Tadashi Yoshino, Tomoko Tanaka, Tsuyoshi Sugamura, Yuriko
Description	Country affiliation: Japan Author Affiliation: Saeki T ( Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.); Sugamura Y ( Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.); Hosokawa M ( Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.); Yoshino T ( Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.); Lim TK ( Malcom Co., Ltd., 4-15-10, Honmachi, Shibuya-ku, Tokyo 151-0071, Japan.); Harada M ( Malcom Co., Ltd., 4-15-10, Honmachi, Shibuya-ku, Tokyo 151-0071, Japan.); Matsunaga T ( Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.); Tanaka T ( Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan. Electronic address: tsuyo@cc.tuat.ac.jp.)
Abstract	This study presents a novel method for CD4 testing based on one-shot large-field imaging. The large-field imaging system was fabricated by a microcavity array and a two-dimensional (2D) photosensor within the desk-top-sized instrument. The microcavity array was employed to separate leukocytes from whole blood based on differences in the size of leukocytes and other blood cells. The large-field imaging system with lower side irradiation enabled acquisition of cell signatures with high signal-to-noise ratio, because the metallic substrate of the microcavity array obstructed excessive excitation light. In this setting, dual-color imaging of CD4(+) and CD8(+) T cells was achieved within the entire image area (64 mm(2)) in 2s. The practical performance of the large-field imaging system was demonstrated by determining the CD4/CD8 ratio in a few microliter of control whole blood as small as those obtained by a finger prick. The CD4/CD8 ratios measured using the large-field imaging system correlated well with those measured by microscopic analysis. These results indicate that our proposed system provides a simple and rapid CD4 testing for the application of HIV/AIDS treatment.
ISSN	09565663
Volume Number	67
e-ISSN	18734235
Journal	Biosensors and Bioelectronics
Language	English
Publisher	Elsevier
Publisher Date	2015-05-15
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biosensing Techniques Cd4-positive T-lymphocytes Pathology Cd8-positive T-lymphocytes Microfluidic Analytical Techniques Acquired Immunodeficiency Syndrome Blood Therapy Cell Separation Humans Signal-to-noise Ratio Tissue Array Analysis Methods Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry

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