NDLI: Layer-by-layer generation of PEG-based regenerable immunosensing surfaces for small-sized analytes.

Please wait, while we are loading the content...

Year: 2017 Volume: 94

Year: 2017 Volume: 92

Year: 2017 Volume: 91

Year: 2017 Volume: 90

Year: 2017 Volume: 89

Year: 2017 Volume: 88

Year: 2017 Volume: 87

Year: 2016 Volume: 90

Year: 2016 Volume: 89

Year: 2016 Volume: 86

Year: 2016 Volume: 85

Year: 2016 Volume: 84

Year: 2016 Volume: 83

Year: 2016 Volume: 82

Year: 2016 Volume: 81

Year: 2016 Volume: 80

Year: 2016 Volume: 79

Year: 2016 Volume: 78

Year: 2016 Volume: 77

Year: 2016 Volume: 76

Year: 2016 Volume: 75

Year: 2015 Volume: 74

Year: 2015 Volume: 73

Year: 2015 Volume: 72

Year: 2015 Volume: 71

Year: 2015 Volume: 70

Year: 2015 Volume: 69

Year: 2015 Volume: 68

Year: 2015 Volume: 67

A new room temperature gas sensor based on pigment-sensitized TiO2 thin film for amines determination.

Sensing based on the motion of enzyme-modified nanorods.

Electrochemical Impedance Spectroscopy (bio)sensing through hydrogen evolution reaction induced by gold nanoparticles.

Fenton fragmentation for faster electrophoretic on chip purification of amplifiable genomic DNA.

Single gold nanoplasmonic sensor for clinical cancer diagnosis based on specific interaction between nucleic acids and protein.

Digital quantification of rolling circle amplified single DNA molecules in a resistive pulse sensing nanopore.

Rapid sandwich ELISA-based in vitro diagnostic procedure for the highly-sensitive detection of human fetuin A.

Nanorods of a new metal-biomolecule coordination polymer showing novel bidirectional electrocatalytic activity and excellent performance in electrochemical sensing.

3D hydrodynamic focusing microfluidics for emerging sensing technologies.

Immobilization of superoxide dismutase on Pt-Pd/MWCNTs hybrid modified electrode surface for superoxide anion detection.

Two-stage microfluidic chip for selective isolation of circulating tumor cells (CTCs).

A true theranostic approach to medicine: towards tandem sensor detection and removal of endotoxin in blood.

Recognition of bacterial lipopolysaccharide using bacteriophage-adhesin-coated long-period gratings.

Whole-cell based label-free capacitive biosensor for rapid nanosize-dependent toxicity detection.

Nanochannel array device operating through Prussian blue nanoparticles for sensitive label-free immunodetection of a cancer biomarker.

Voltage-controlled fluorescence switching of a single redox protein.

Highly selective and sensitive determination of dopamine by the novel molecularly imprinted poly(nicotinamide)/CuO nanoparticles modified electrode.

Temperature-controlled microintaglio printing for high-resolution micropatterning of RNA molecules.

Surface modification of silicon dioxide, silicon nitride and titanium oxynitride for lactate dehydrogenase immobilization.

A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes.

Multiplexed gold nanorod array biochip for multi-sample analysis.

Electrochemical performance and detection of 8-Hydroxy-2'-deoxyguanosine at single-stranded DNA functionalized graphene modified glassy carbon electrode.

Lytic enzymes as selectivity means for label-free, microfluidic and impedimetric detection of whole-cell bacteria using ALD-Al2O3 passivated microelectrodes.

Alzheimer's disease biomarkers detection in human samples by efficient capturing through porous magnetic microspheres and labelling with electrocatalytic gold nanoparticles.

High-performance beating pattern function of human induced pluripotent stem cell-derived cardiomyocyte-based biosensors for hERG inhibition recognition.

Simultaneous amperometric detection of ascorbic acid and antioxidant capacity in orange, blueberry and kiwi juice, by a telemetric system coupled with a fullerene- or nanotubes-modified ascorbate subtractive biosensor.

In silico designed nanoMIP based optical sensor for endotoxins monitoring.

Human T cells monitored by impedance spectrometry using field-effect transistor arrays: a novel tool for single-cell adhesion and migration studies.

Aptamer/Au nanoparticles/cobalt sulfide nanosheets biosensor for 17ß-estradiol detection using a guanine-rich complementary DNA sequence for signal amplification.

A smartphone-based colorimetric reader for bioanalytical applications using the screen-based bottom illumination provided by gadgets.

ZnO-CuO composite matrix based reagentless biosensor for detection of total cholesterol.

Detection of CTX-II in serum and urine to diagnose osteoarthritis by using a fluoro-microbeads guiding chip.

Fast and sensitive optical toxicity bioassay based on dual wavelength analysis of bacterial ferricyanide reduction kinetics.

A microfluidic nano-biosensor for the detection of pathogenic Salmonella.

Nanoplasmonic biosensor: coupling electrochemistry to localized surface plasmon resonance spectroscopy on nanocup arrays.

Sensitive electrical detection of human prion proteins using field effect transistor biosensor with dual-ligand binding amplification.

Integrated potentiostat for electrochemical sensing of urinary 3-hydroxyanthranilic acid with molecularly imprinted poly(ethylene-co-vinyl alcohol).

Optical waveguide sensor based on silica nanotube arrays for label-free biosensing.

Fluorescent gold nanoclusters based photoelectrochemical sensors for detection of H2O2 and glucose.

Detection of parathion and patulin by quartz-crystal microbalance functionalized by the photonics immobilization technique.

Fast and continuous microorganism detection using aptamer-conjugated fluorescent nanoparticles on an optofluidic platform.

Switchable aptamers for biosensing and bioseparation of viruses (SwAps-V).

Mercury (II) sensor based on monitoring dissociation rate of the trans-acting factor MerR from cis-element by surface plasmon resonance.

Highly selective and sensitive detection of ß-agonists using a surface plasmon resonance sensor based on an alkanethiol monolayer functionalized on a Au surface.

Simple and rapid CD4 testing based on large-field imaging system composed of microcavity array and two-dimensional photosensor.

A novel layer-by-layer assembled multi-enzyme/CNT biosensor for discriminative detection between organophosphorus and non-organophosphrus pesticides.

Analysis of telomerase activity based on a spired DNA tetrahedron TS primer.

Droplet-based microfluidics for high-throughput screening of a metagenomic library for isolation of microbial enzymes.

Synthesis of short graphene oxide nanoribbons for improved biomarker detection of Parkinson's disease.

Yeast-based self-organized hybrid bio-silica sol-gels for the design of biosensors.

Layer-by-layer generation of PEG-based regenerable immunosensing surfaces for small-sized analytes.

Real-time ligation chain reaction for DNA quantification and identification on the FO-SPR.

Bio-nanogate controlled enzymatic reaction for virus sensing.

Drug response of captured BT20 cells and evaluation of circulating tumor cells on a silicon nanowire platform.

A facile and sensitive detection of organophosphorus chemicals by rapid aggregation of gold nanoparticles using organic compounds.

Fabrication of copper nanoparticles decorated multiwalled carbon nanotubes as a high performance electrochemical sensor for the detection of neotame.

Rapid and molecular selective electrochemical sensing of phthalates in aqueous solution.

Homogeneous detection of caspase-3 using intrinsic fluorescence resonance energy transfer (iFRET).

Biosensing enhancement of dengue virus using microballoon mixers on centrifugal microfluidic platforms.

Development of the automated circulating tumor cell recovery system with microcavity array.

Nanoplasmonic biosensor: detection and amplification of dual bio-signatures of circulating tumor DNA.

A novel paper-based assay for the simultaneous determination of Rh typing and forward and reverse ABO blood groups.

Glucose-driven chemo-mechanical autonomous drug-release system with multi-enzymatic amplification toward feedback control of blood glucose in diabetes.

An improved sensitive assay for the detection of PSP toxins with neuroblastoma cell-based impedance biosensor.

A reusable magnetic graphene oxide-modified biosensor for vascular endothelial growth factor detection in cancer diagnosis.

A smart DNA sensing system for detecting methicillin-resistant Staphylococcus aureus using modified nanoparticle probes.

Microfluidic optoelectronic sensor for salivary diagnostics of stomach cancer.

Quantitative impedimetric NPY-receptor activation monitoring and signal pathway profiling in living cells.

Neurodegeneration through oxidative stress: monitoring hydrogen peroxide induced apoptosis in primary cells from the subventricular zone of BALB/c mice using field-effect transistors.

Highly sensitive and rapid determination of Escherichia coli O157:H7 in minced beef and water using electrocatalytic gold nanoparticle tags.

Ultrasensitive SERS detection of mercury based on the assembled gold nanochains.

Paper based platform for colorimetric sensing of dissolved NH3 and CO2.

A droplet-based fluorescence polarization immunoassay (dFPIA) platform for rapid and quantitative analysis of biomarkers.

Development of electrochemical methods to enzymatically detect traumatic brain injury biomarkers.

A surface acoustic wave bio-electronic nose for detection of volatile odorant molecules.

Parylene-based flexible neural probes with PEDOT coated surface for brain stimulation and recording.

Fish stress become visible: a new attempt to use biosensor for real-time monitoring fish stress.

DNA-based biosensors for Hg(2+) determination by polythymine-methylene blue modified electrodes.

An impedance immunosensor based on low-cost microelectrodes and specific monoclonal antibodies for rapid detection of avian influenza virus H5N1 in chicken swabs.

Ultrasensitive detection of streptomycin using flow injection analysis-electrochemical quartz crystal nanobalance (FIA-EQCN) biosensor.

-Amylase sensor based on the degradation of oligosaccharide hydrogel films monitored with a quartz crystal sensor.

A novel electrochemical method to detect theophylline utilizing silver ions captured within abasic site-incorporated duplex DNA.

A paper strip based non-invasive glucose biosensor for salivary analysis.

Evaluation of direct versus multi-layer passivation and capture chemistries for nanoparticle-based biosensor applications.

Droplet-based immunoassay on a 'sticky' nanofibrous surface for multiplexed and dual detection of bacteria using smartphones.

A fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) for the detection of mecA gene sequence of Staphylococcus aureus.

Detection of Bacillus anthracis spores by super-paramagnetic lateral-flow immunoassays based on 'Road Closure'.

Printed and flexible biosensor for antioxidants using interdigitated ink-jetted electrodes and gravure-deposited active layer.

New ZnO nanostructures as non-enzymatic glucose biosensors.

Real-time multianalyte biosensors based on interference-free multichannel monolithic quartz crystal microbalance.

Tailored carbon nanotube immunosensors for the detection of microbial contamination.

Noise spectroscopy as an efficient tool for impedance based sub-femtomolar toxin detection in complex mixture using nanoporous silicon oxide.

A novel 96-well multielectrode array based impedimetric monitoring platform for comparative drug efficacy analysis on 2D and 3D brain tumor cultures.

Chemiluminescent imaging of transpired ethanol from the palm for evaluation of alcohol metabolism.

The microfluidic chip module for the detection of murine norovirus in oysters using charge switchable micro-bead beating.

A biomimetic sensor for the detection of lead in water.

An unconventional approach to impedance microbiology: detection of culture media conductivity variations due to bacteriophage generated lyses of host bacteria.

Peptide conjugated chitosan foam as a novel approach for capture-purification and rapid detection of hapten--example of ochratoxin A.

Quantification of rolling circle amplified DNA using magnetic nanobeads and a Blu-ray optical pick-up unit.

Olfactory biosensor for insect semiochemicals analysis by impedance sensing of odorant-binding proteins on interdigitated electrodes.

Highly sensitive silicon nanowire biosensor with novel liquid gate control for detection of specific single-stranded DNA molecules.

Low-cost telemedicine device performing cell and particle size measurement based on lens-free shadow imaging technology.

In vivo continuous and simultaneous monitoring of brain energy substrates with a multiplex amperometric enzyme-based biosensor device.

Magnetic bead droplet immunoassay of oligomer amyloid ß for the diagnosis of Alzheimer's disease using micro-pillars to enhance the stability of the oil-water interface.

Sensitive biosensor based on recombinant PP1 for microcystin detection.

Antithyroid drug detection using an enzyme cascade blocking in a nanoparticle-based lab-on-a-chip system.

Detection and classification of natural odors with an in vivo bioelectronic nose.

Detection of the cyanobacterial toxin, microcystin-LR, using a novel recombinant antibody-based optical-planar waveguide platform.

A reusable robust radio frequency biosensor using microwave resonator by integrated passive device technology for quantitative detection of glucose level.

Surface-enhanced Raman spectroscopy detection of dopamine by DNA Targeting amplification assay in Parkisons's model.

Development and application of a new electronic nose instrument for the detection of colorectal cancer.

Preface. Biosensors 2014.

Year: 2015 Volume: 66

Year: 2015 Volume: 65

Year: 2015 Volume: 64

Year: 2015 Volume: 63

Year: 2014 Volume: 62

Year: 2014 Volume: 61

Year: 2014 Volume: 60

Year: 2014 Volume: 59

Year: 2014 Volume: 58

Year: 2014 Volume: 57

Year: 2014 Volume: 56

Year: 2014 Volume: 55

Year: 2014 Volume: 54

Year: 2014 Volume: 53

Year: 2014 Volume: 52

Year: 2014 Volume: 51

Year: 2013 Volume: 50

Year: 2013 Volume: 49

Year: 2013 Volume: 48

Year: 2013 Volume: 47

Year: 2013 Volume: 46

Year: 2013 Volume: 45

Year: 2013 Volume: 44

Year: 2013 Volume: 43

Year: 2013 Volume: 42

Year: 2013 Volume: 41

Year: 2013 Volume: 40

Year: 2013 Volume: 39

Year: 2012 Volume: 38

Year: 2012 Volume: 37

Year: 2012 Volume: 36

Year: 2012 Volume: 35

Year: 2012 Volume: 34

Year: 2012 Volume: 33

Year: 2012 Volume: 32

Year: 2012 Volume: 31

Year: 2011 Volume: 30

Year: 2011 Volume: 29

Year: 2011 Volume: 28

Year: 2011 Volume: 27

Year: 2011 Volume: 26

Year: 2010 Volume: 26

Year: 2010 Volume: 25

Year: 2009 Volume: 25

Year: 2009 Volume: 24

Year: 2008 Volume: 24

Year: 2008 Volume: 23

Year: 2007 Volume: 23

Year: 2007 Volume: 22

Year: 2006 Volume: 21

Year: 2005 Volume: 21

Layer-by-layer generation of PEG-based regenerable immunosensing surfaces for small-sized analytes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Huebner, Maria Ben Haddada, Maroua Boujday, Souhir Knopp, Dietmar Méthivier, Christophe Niessner, Reinhard
Description	Country affiliation: Germany Author Affiliation: Huebner M ( Chair for Analytical Chemistry and Institute of Hydrochemistry, Technische Universität München, Marchioninistr. 17, Munich, Germany.); Ben Haddada M ( Sorbonne Universités, UPMC Univ Paris 6, UMR CNRS 7197, Laboratoire de Réactivité de Surface, F75005 Paris, France); Méthivier C ( Sorbonne Universités, UPMC Univ Paris 6, UMR CNRS 7197, Laboratoire de Réactivité de Surface, F75005 Paris, France); Niessner R ( Chair for Analytical Chemistry and Institute of Hydrochemistry, Technische Universität München, Marchioninistr. 17, Munich, Germany.); Knopp D ( Chair for Analytical Chemistry and Institute of Hydrochemistry, Technische Universität München, Marchioninistr. 17, Munich, Germany. Electronic address: dietmar.knopp@ch.tum.de.); Boujday S ( Sorbonne Universités, UPMC Univ Paris 6, UMR CNRS 7197, Laboratoire de Réactivité de Surface, F75005 Paris, France)
Abstract	Small molecules (haptens) like pharmaceuticals or peptides can serve as targets for antibody binding in competitive immunoassay-based flow-through assays. In this work, a strategy for preparing polyethylene glycol (PEG) coatings for subsequent hapten immobilization on glass-type silica surfaces is presented and characterized in detail. Two substrates bearing terminal silanol groups were utilized, a glass slide and a silicon wafer. First, surfaces were thoroughly cleaned and pretreated to generate additional silanol groups. Then, a silane layer with terminal epoxy groups was created using 3-glycidyloxypropyltrimethoxysilane (GOPTS). Epoxy groups were used to bind a layer of diamino-poly(ethylene glycol) (DAPEG) with terminal amino groups. Finally, the low molecular weight compound diclofenac was bound to the surface to be used as model ligand for competitive biosensing of haptens. The elementary steps were characterized using atomic force microscopy (AFM), water contact angle measurement, grazing-angle attenuated total reflection (GA-ATR) FT-IR spectroscopy, and X-ray photoelectron spectroscopy (XPS). The data collected using these techniques have confirmed the successive grafting of the molecular species, evidencing, that homogeneous monolayers were created on the silica surfaces and validated the proposed mechanism of functionalization. The resulting surfaces were used to investigate polyclonal anti-diclofenac antibodies recognition and reversibility using quartz crystal microbalance with dissipation (QCM-D) measurements or an automated flow-through immunoassay with chemiluminescence (CL) read-out. For both techniques, recognition and reversibility of the antibody binding were observed. The stability of sensors over time was also assessed and no decrease in CL response was observed upon 14 days in aqueous solution. The herein presented strategy for surface functionalization can be used in the future as reproducible and reusable universal platform for hapten biosensors.
ISSN	09565663
Volume Number	67
e-ISSN	18734235
Journal	Biosensors and Bioelectronics
Language	English
Publisher	Elsevier
Publisher Date	2015-05-15
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Antibodies Isolation & Purification Biosensing Techniques Haptens Chemistry Quartz Crystal Microbalance Techniques Immunology Luminescence Microscopy, Atomic Force Polyethylene Glycols Silicon Dioxide Spectroscopy, Fourier Transform Infrared Surface Properties Water Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in