NDLI: Identification of ecotropic proviral sequences in inbred mouse strains with a cloned subgenomic DNA fragment.

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Identification of the slow reacting substances from cat paws.

Products of reaction catalyzed by purified rat liver guanylate cyclase determined by 31p NMR spectroscopy.

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Identification of ecotropic proviral sequences in inbred mouse strains with a cloned subgenomic DNA fragment.

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Identification of ecotropic proviral sequences in inbred mouse strains with a cloned subgenomic DNA fragment.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bryan, T. Chan, H. W. Staal, S. P. Rowe, W. P. Moore, J. L. Martin, M. A.
Abstract	A specific probe for detecting ecotropic murine leukemia virus sequences was constructed by cloning a 500-base-pair DNA segment, corresponding to a portion of the env region of the AKR ecotropic virus, in a pBR322/Escherichia coli K-12 host/vector system. This probe was used to screen the cellular DNAs of six inbred strains of mice for the presence of ecotropic retroviral DNA sequences by the Southern blot hybridization procedure. Three copies of ecotropic viral DNA were detected in AKR/N (a high-ecotropic virus strain) and two were found in BALB/c (a low-ecotropic virus strain) DNAs. As expected, no sequences reactive with this probe were found in NFS mouse DNA (a virus-negative strain). However, cellular DNA sequences that reacted strongly with the ecotropic-specific DNA probe were detected in certain NZB, C57L, and 129 mice (all virus-negative strains). In contrast to the reactive sequences in AKR and BALB/c, the reactions were chiefly associated with EcoRI segments that were subgenomic in size.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	10
Volume Number	77
Language	English
Publisher	National Academy of Sciences
Publisher Date	1980-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	DNA, Viral Genetics Genes, Viral Leukemia Virus, Murine Mice, Inbred Strains Microbiology Animals DNA, Recombinant Mice Nucleic Acid Hybridization Species Specificity Virus Replication Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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