NDLI: Regulation of simian virus 40 early transcription in vitro by a purified tumor antigen.

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Identification of the slow reacting substances from cat paws.

Products of reaction catalyzed by purified rat liver guanylate cyclase determined by 31p NMR spectroscopy.

Nucleotide sequence of a cloned structural gene coding for a precursor of pancreatic somatostatin.

Regulation of insulin receptors: evidence for involvement of an endocytotic internalization pathway.

Complementation of arylsulfatase A in somatic hybrids of metachromatic leukodystrophy and multiple sulfatase deficiency disorder fibroblasts.

Synthesis and biological actions of prosomatostatin.

Immunoreactive dynorphin in pituitary and brain.

Catecholamine biosynthetic enzymes are expressed in replicating cells of the peripheral but not the central nervous system.

Direct observation of the torsional dynamics of DNA and RNA by picosecond spectroscopy.

Rate-limiting steps in RNA chain initiation.

5-fluorouracil-methotrexate synergy: enhancement of 5-fluorodeoxyridylate binding to thymidylate synthase by dihydropteroylpolyglutamates.

NH2-terminal amino acid sequence of human fibroblast interferon.

Variations in properties of virus released from morphologically different cell lines transformed in vitro by Friend leukemia virus.

Elongation of primed DNA templates by eukaryotic DNA polymerases.

Progesterone inhibition of uterine nuclear estrogen receptor: dependence on RNA and protein synthesis.

Altered enolase in aged Turbatrix aceti results from conformational changes in the enzyme.

Oligosaccharide motion in erythrocyte membranes investigated by picosecond fluorescence polarization and microsecond dichroism of an optical probe.

Fast axonal transport in the presence of high Ca2+: evidence that microtubules are not required.

Metabolism of native and of lactosylated human low density lipoprotein: evidence for two pathways for catabolism of exogenous proteins in rat hepatocytes.

Differential efficacies of human type I and type II interferons as antiviral and antiproliferative agents.

Spontaneous production of human interferon.

Human leukocyte interferon: structural and biological relatedness to adrenocorticotropic hormone and endorphins.

Potent stimulation of vascular endothelial cell growth by differentiated 3T3 adipocytes.

Polymorphism of human B-cell alloantigens: evidence for three loci within the HLA system.

Modulation of cyclic AMP metabolism by S-adenosylhomocysteine and S-3-deazaadenosylhomocysteine in mouse lymphocytes.

Sendai virus utilizes specific sialyloligosaccharides as host cell receptor determinants.

Regulation of simian virus 40 early transcription in vitro by a purified tumor antigen.

Adrenal glucocorticoid permissive regulation of muscle glycogenolysis: action on protein phosphatase(s) and its inhibitor(s).

(R)-mevalonate excretion in human and rat urines.

beta-Endorphin: characteristics of binding sites in rabbit spinal cord.

Structure of endogenous murine leukemia virus DNA in mouse genomes.

Identification of ecotropic proviral sequences in inbred mouse strains with a cloned subgenomic DNA fragment.

Catabolite repression in Escherichia coli mutants lacking cyclic AMP receptor protein.

Characterization of a receptor-like protein for 1,25-dihydroxyvitamin D3 in rat skin.

Comparison of two delta-crystallin genes in the chicken.

Calcium-dependent hormonal regulation of amino acid transport and cyclic AMP accumulation in rat hepatocyte monolayer cultures.

Effects of combined glutamine and serum deprivation on glucose control of hexose transport in mammalian fibroblast cultures.

Platelet-derived growth factor stimulates low density lipoprotein receptor activity in cultured human fibroblasts.

Inhibiting the onset of hormone-induced desentiziation of viable thymocytes by N alpha-tosyl-L-lysine chloromethyl ketone.

Insulin and serum increase the number of receptors for vasopressin in a kidney-derived line of cells grown in a defined medium.

Growth of T-lymphoma cells in serum-free medium: lack of involvement of the cyclic AMP pathway in long-term cultures.

Regulation of Tn5 transposition in Salmonella typhimurium.

Antigen-liposome modification of target cells as a method to alter their susceptibility to lysis by cytotoxic T lymphocytes.

Frequency and levels of antibodies to Epstein-Barr virus-specific DNase are elevated in patients with nasopharyngeal carcinoma.

Cytotypically specific transfecting activity of DNA from C57BL/Ka mouse cells producing thymotropic in contrast to nonthymotropic retroviruses.

An endonuclease isolated from Epstein-Barr virus-producing human lymphoblastoid cells.

Characterization of Y73, an avian sarcoma virus: a unique transforming gene and its product, a phosphopolyprotein with protein kinase activity.

Avermectin B1a, a paralyzing anthelmintic that affects interneurons and inhibitory motoneurons in Ascaris.

Facilitation of membrane electrical excitability in Drosophila.

Differentiation of delta and mu opiate receptor localizations by light microscopic autoradiography.

Electrical inhibition of Purkinje cells in the cerebellum of the rat.

An activation factor of liver phosphofructokinase.

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A human soluble suppressor factor affecting lymphocyte responses in vitro.

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Evolutionary origins of vertebrate hormones: substances similar to mammalian insulins are native to unicellular eukaryotes.

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Expression of ribosomal proteins during Drosophila early development.

Organization of actin gene sequences in the sea urchin: molecular cloning of an intron-containing DNA sequence coding for a cytoplasmic actin.

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Untranslated mRNA for a chorion protein of Drosophila melanogaster accumulates transiently at the onset of specific gene amplification.

Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene.

Immunoglobulin V/J recombination is accompanied by deletion of joining site and variable region segments.

Cloning and partial nucleotide sequence of human immunoglobulin mu chain cDNA from B cells and mouse-human hybridomas.

Site specificity of mutations arising in dysgenic hybrids of Drosophila melanogaster.

Self-association and base pairing of guanosine, cytidine, adenosine, and uridine in dimethyl sulfoxide solution measured by 15N nuclear magnetic resonance spectroscopy.

Geminate recombination of O2 and hemoglobin.

Parathyroid hormone alters collagen synthesis and procollagen mRNA levels in fetal rat calvaria.

Reiteration frequency mapping: analysis of repetitive sequence organization within cloned DNA fragments containing the human initiator methionine tRNA gene.

Pentaribonucleotides of mixed sequence are synthesized and efficiently prime de novo DNA chain starts in the T4 bacteriophage DNA replication system.

Relation between enzymic catalysis and energy coupling.

In vitro synthesis of 1 alpha,25-dihydroxycholecalciferol and 24,25-dihydroxycholecalciferol by isolated calvarial cells.

Immunological identification of the human erythrocyte glucose transporter.

Subunit neighbor interactions in enzyme kinetics: half-of-the-sites reactivity in a dimer.

Cytoplasmic processing of myosin heavy chain messenger RNA: evidence provided by using a recombinant DNA plasmid.

Exons encode functional and structural units of chicken lysozyme.

Evidence for presence of an internal thiolester bond in third component of human complement.

Dihydrofolate reductase: thymidylate synthase, a bifunctional polypeptide from Crithidia fasciculata.

Topographic studies of Torpedo acetylcholine receptor subunits as a transmembrane complex.

Calcium-regulated phosphorylation in synaptosomal cytosol: dependence on calmodulin.

Studies on the active center of D- and L-lactate dehydrogenases using oxamate-diaminohexyl-Sepharose affinity chromatography.

Hormone-induced guanyl nucleotide binding and activation of adenylate cyclase in the Leydig cell.

Isopentenyladenine as a mediator of mevalonate-regulated DNA replication.

Affinity chromatography in nonionic detergent solutions.

Purification of myosin light chain kinase from bovine cardiac muscle.

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Human platelet basic protein associated with antiheparin and mitogenic activities: purification and partial characterization.

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Malignancies of metastatic murine lymphosarcoma cell lines and clones correlate with decreased cell surface display of RNA tumor virus envelope glycoprotein gp70.

Amiloride inhibits murine erythroleukemia cell differentiation: evidence for a Ca2+ requirement for commitment.

Transcription units of chicken ovalbumin gene observed after injection of cloned complete genes into Xenopus oocyte nuclei.

Manufacture of diploid/tetraploid chimeric mice.

Association of ribosomal genes in the fibrillar center of the nucleolus: a factor influencing translocation and nondisjunction in the human meiotic oocyte.

Rate of spontaneous mutation at human loci encoding protein structure.

Switches in macromolecular synthesis during induction of competence for transformation of Streptococcus sanguis.

Postreplication repair and the susceptibility of Chinese hamster cells to cytotoxic and mutagenic effects of alkylating agents.

Evidence for unequal crossing over within the mouse T/t complex.

Molecular cloning of a human histocompatibility antigen cDNA fragment.

Quantitation of mRNAs during mouse spermatogenesis: protamine-like histone and phosphoglycerate kinase-2 mRNAs increase after meiosis.

Immunosuppression by human plasma alpha 1-acid glycoprotein: importance of the carbohydrate moiety.

Cell surface antigens of human malignant melanoma: definition of six antigenic systems with mouse monoclonal antibodies.

Autoradiographic method for measuring the ischemic myocardium at risk: effects of verapamil on infarct size aftr experimental coronary artery occlusion.

delta Agent: association of delta antigen with hepatitis B surface antigen and RNA in serum of delta-infected chimpanzees.

Human lysosomes can be purified from diploid skin fibroblasts by free-flow electrophoresis.

Erythrocyte choline concentrations are elevated in manic patients.

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Constitutive uncoupling of pathways of gene expression that control growth and differentiation in myeloid leukemia: a model for the origin and progression of malignancy.

Cord-blood tyrosine levels in the full-term phenylketonuric fetus and the 'justification hypothesis'.

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Rapid taste-aversion learning by an isolated molluscan central nervous system.

Elongating nerve fibers are guided by a pathway of material released from embryonic nonneuronal cells.

Identification and localization of glucagon-related peptides in rat brain.

Specific stimulated uptake of acetylcholine by Torpedo electric organ synaptic vesicles.

Resurgence of responding after the cessation of response-independent reinforcement.

Purification of kidney epithelial cell growth inhibitors.

Cellular interactions in the generation of cytolytic T lymphocyte responses: role of Ia-positive splenic adherent cells in presentation in H-2 antigen.

Purification and some characteristics of human T-cell growth factor from phytohemagglutinin-stimulated lymphocyte-conditioned media.

ATP depletion as a consequence of adenosine deaminase inhibition in man.

Nuclear Overhauser effect in specifically deuterated macromolecules: NMR assay for unusual base pairing in transfer RNA.

Identification of proteolytically resistant domains of human erythrocyte spectrin.

Immunocytochemical localization of the sex steroid-binding protein of plasma in tissues of the adult monkey Macaca nemestrina.

Nucleotide sequence of the thrA gene of Escherichia coli.

New molecular forms of human liver alcohol dehydrogenase: isolation and characterization of ADHIndianapolis.

Absolute requirement of glucocorticoid for expression of the casein gene in the presence of prolactin.

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Regulation of simian virus 40 early transcription in vitro by a purified tumor antigen.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Myers, R. Robbins, A. Rio, D. Tjian, R.
Abstract	Cloned DNA templates were used to direct the transcription of early and late simian virus 40 (SV40) genes by a cell-free RNA-synthesizing system. Transcription by RNA polymerase II was sensitive to low levels of alpha-amanitin and completely dependent on exogenously added DNA template. RNA products of discrete lengths were efficiently synthesized when transcription was directed by DNA restriction fragments containing promoter sequences for either early or late genes of SV40. Addition of the D2 tumor antigen to the template DNA inhibited transcription originating from the SV40 early promoter. In contrast, the D2 protein had little or no effect on the transcription from SV40 or adenovirus 2 (Ad2) late promoter sequences. When a mixture of cloned DNA containing SV40 early promoter and Ad2 late promoter was used to direct RNA synthesis, the D2 protein specifically inhibited the synthesis of SV40 early genes but not that of Ad2 late sequences. The D2 DNA binding protein also had no effect on the transcription directed by SV40 mutant templates that contain an intact early promoter sequence but lack specific tumor-antigen binding sites. We have confirmed that, under the conditions of the transcription assay, the D2 protein binds and interacts specifically with its recognition sites on wild-type template DNAs but fails to bind to mutant or Ad2 DNA templates that lack sequences containing SV40 tumor-antigen binding sites. These findings provide evidence that a direct interaction between tumor antigen and its specific binding sites on DNA is the mechanism by which the SV40 A gene autoregulates its transcription.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	10
Volume Number	77
Language	English
Publisher	National Academy of Sciences
Publisher Date	1980-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Antigens, Neoplasm Antigens, Viral Simian Virus 40 Genetics Transcription, Genetic Viral Proteins Antigens, Viral, Tumor Binding Sites DNA, Viral Metabolism Gene Expression Regulation Operon Immunology Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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