NDLI: The number of positively charged amino acids in the basic domain of Tat is critical for trans-activation and complex formation with TAR RNA.

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The number of positively charged amino acids in the basic domain of Tat is critical for trans-activation and complex formation with TAR RNA.

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The number of positively charged amino acids in the basic domain of Tat is critical for trans-activation and complex formation with TAR RNA.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Delling, U. Barnett, R. Sumner-smith, M. Reid, L. Roy, S. Rosen, C. A. Sonenberg, N.
Description	Author Affiliation: Delling U ( Department of Biochemistry, McGill University, Canada.);
Abstract	The basic domain of Tat is required for trans-activation of viral gene expression. We have performed scanning peptide studies to demonstrate that only this domain is capable of binding to the TAR RNA stem-loop. Strikingly, the basic domain of the other human immunodeficiency virus trans-acting factor, Rev, but no other region, is also capable of binding to TAR. Peptide derivatives of Tat do not require the highly conserved glutamine residue at position 54 for TAR binding, since it may be substituted or deleted. In addition, the two lysine residues may be replaced by arginines. Analysis of binding and trans-activation demonstrated that homopolymers of arginine can completely substitute for the basic domain. Such homopolymers have high affinity for wild-type TAR RNA and lower affinity for mutant TAR. Homopolymers of six to nine arginines substituting for the basic domain of Tat enable full trans-activation in vivo. Homopolymers of at least seven arginines are required for detectable in vitro complex formation, although approximately 30% trans-activation is achieved with a mutant Tat containing only five arginines.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	14
Volume Number	88
Language	English
Publisher	National Academy of Sciences
Publisher Date	1991-08-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Arginine Gene Products, Tat Genetics HIV Long Terminal Repeat HIV-1 RNA, Viral Transcriptional Activation Amino Acid Sequence Binding Sites Chloramphenicol O-Acetyltransferase Metabolism Gene Products, Rev Genes, Tat HeLa Cells Kinetics Molecular Sequence Data Mutagenesis, Site-Directed Transfection Rev Gene Products, Human Immunodeficiency Virus Tat Gene Products, Human Immunodeficiency Virus Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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