NDLI: Molecular cloning and characterization of the yeast gene for squalene synthetase.

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Molecular cloning and characterization of the yeast gene for squalene synthetase.

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Molecular cloning and characterization of the yeast gene for squalene synthetase.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Jennings, S. M. Tsay, Y. H. Fisch, T. M. Robinson, G. W.
Description	Author Affiliation: Jennings SM ( Department of Cellular Biology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, NJ 08543-4000.);
Abstract	Squalene synthetase (farnesyl-diphosphate: farnesyl-diphosphate farnesyltransferase, EC 2.5.1.21) is a critical branch point enzyme of isoprenoid biosynthesis that is thought to regulate the flux of isoprene intermediates through the sterol pathway. The structural gene for this enzyme was cloned from the yeast Saccharomyces cerevisiae by functional complementation of a squalene synthetase-deficient erg9 mutant. Identification of this ERG9 clone was confirmed by genetic linkage analysis in yeast and expression of enzyme activity in Escherichia coli. The predicted squalene synthetase polypeptide of 444 amino acids (Mr, 51,753) lacks significant homology to known protein sequences, except within a region that may represent a prenyl diphosphate (substrate) binding site. The ERG9-encoded protein contains a PEST consensus motif (rich in proline, glutamic acid, serine, and threonine) present in many proteins with short cellular half-lives. Modeling of the protein suggests that it contains at least one, and possibly two, membrane-spanning domains. Disruption of the chromosomal squalene synthetase coding region by insertional mutagenesis indicates that ERG9 is a single copy gene that is essential for cell growth in yeast.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	14
Volume Number	88
Language	English
Publisher	National Academy of Sciences
Publisher Date	1991-08-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Farnesyl-Diphosphate Farnesyltransferase Genetics Genes, Fungal Saccharomyces Cerevisiae Amino Acid Sequence Cloning, Molecular Escherichia Coli Metabolism Gene Expression Gene Library Genetic Complementation Test Microsomes Enzymology Molecular Sequence Data Molecular Weight Nucleic Acid Hybridization Plasmids Protein Conformation Recombinant Proteins Restriction Mapping Sequence Homology, Nucleic Acid Comparative Study Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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