NDLI: Evidence for alternating head catalysis by kinesin during microtubule-stimulated ATP hydrolysis.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1994 Volume: 91 Issue: 26

Year: 1994 Volume: 91 Issue: 26 Suppl

Year: 1994 Volume: 91 Issue: 25

Year: 1994 Volume: 91 Issue: 24

Year: 1994 Volume: 91 Issue: 23

Year: 1994 Volume: 91 Issue: 22

Year: 1994 Volume: 91 Issue: 21

Year: 1994 Volume: 91 Issue: 20

Year: 1994 Volume: 91 Issue: 19

Year: 1994 Volume: 91 Issue: 18

Year: 1994 Volume: 91 Issue: 17

Year: 1994 Volume: 91 Issue: 16

Year: 1994 Volume: 91 Issue: 15

Analytic inversion in synthetic aperture radar.

A decentralized process for finding equilibria given by linear equations.

Genetic variability for stomatal conductance in Pima cotton and its relation to improvements of heat adaptation.

Photosystem II reaction center damage and repair cycle: chloroplast acclimation strategy to irradiance stress.

The history of a genetic system.

RNA template-directed RNA synthesis by T7 RNA polymerase.

Kinetic characterization of intramolecular and intermolecular hammerhead RNAs with stem II deletions.

Tumor necrosis factor alpha is an autocrine growth factor for normal human B cells.

Inhibition of growth of OV-1063 human epithelial ovarian cancer xenografts in nude mice by treatment with luteinizing hormone-releasing hormone antagonist SB-75.

Prolactin activates the interferon-regulated p91 transcription factor and the Jak2 kinase by tyrosine phosphorylation.

Structural basis of asymmetry in the human immunodeficiency virus type 1 reverse transcriptase heterodimer.

Identification of immunosuppressant-induced apoptosis in a murine B-cell line and its prevention by bcl-x but not bcl-2.

Wheat acetyl-coenzyme A carboxylase: cDNA and protein structure.

Linkage of mating-type loci distinguishes bipolar from tetrapolar mating in basidiomycetous smut fungi.

Proposed three-dimensional structure for the cellular prion protein.

Overexpression of ERBB2 in human mammary epithelial cells signals inhibition of transcription of the E-cadherin gene.

CD8+ T lymphocytes of African green monkeys secrete an immunodeficiency virus-suppressing lymphokine.

Three-dimensional structure of a transglutaminase: human blood coagulation factor XIII.

Tempo and mode in evolution.

Tempo, mode, the progenote, and the universal root.

Phylogeny from function: evidence from the molecular fossil record that tRNA originated in replication, not translation.

Disparate rates, differing fates: tempo and mode of evolution changed from the Precambrian to the Phanerozoic.

Proterozoic and early Cambrian protists: evidence for accelerating evolutionary tempo.

Late Precambrian bilaterians: grades and clades.

The role of extinction in evolution.

Tempo and mode in the macroevolutionary reconstruction of Darwinism.

Morphological evolution through complex domains of fitness.

Tempo and mode in human evolution.

Molecular genetics of speciation and human origins.

Rates and patterns of chloroplast DNA evolution.

The superoxide dismutase molecular clock revisited.

Dynamics of adaptation and diversification: a 10,000-generation experiment with bacterial populations.

How can the low levels of DNA sequence variation in regions of the drosophila genome with low recombination rates be explained?

Genome structure and evolution in Drosophila: applications of the framework P1 map.

Error-prone replication of repeated DNA sequences by T7 DNA polymerase in the absence of its processivity subunit.

The absence of IgE antibody-mediated augmentation of immune responses in CD23-deficient mice.

Human growth hormone (hGH) secretion in milk of goats after direct transfer of the hGH gene into the mammary gland by using replication-defective retrovirus vectors.

Rearrangement of the histone H2A C-terminal domain in the nucleosome.

Receptor to nucleus signaling by prolactin and interleukin 2 via activation of latent DNA-binding factors.

Molecular cloning of the human nucleotide-excision-repair gene ERCC4.

Evidence for alternating head catalysis by kinesin during microtubule-stimulated ATP hydrolysis.

Snapshot blotting: transfer of nucleic acids and nucleoprotein complexes from electrophoresis gels to grids for electron microscopy.

Participation of cyclin A in Myc-induced apoptosis.

Yeast Srp1p has homology to armadillo/plakoglobin/beta-catenin and participates in apparently multiple nuclear functions including the maintenance of the nucleolar structure.

Molecular phylogeny of the Anopheles gambiae complex suggests genetic introgression between principal malaria vectors.

A circularly permuted recombinant interleukin 4 toxin with increased activity.

Decreased release of gonadotropin-releasing hormone during the preovulatory midcycle luteinizing hormone surge in normal women.

Frog diazepam-binding inhibitor: peptide sequence, cDNA cloning, and expression in the brain.

DNA-dependent protein kinase (Ku protein-p350 complex) assembles on double-stranded DNA.

A carbonic anhydrase from the archaeon Methanosarcina thermophila.

Expression of wild-type and mutant simian virus 40 large tumor antigens in villus-associated enterocytes of transgenic mice.

Isolation of the bile canalicular actin-myosin II motor.

The A1 and A1B proteins of heterogeneous nuclear ribonucleoparticles modulate 5' splice site selection in vivo.

Male ornament size as a reliable cue to enhanced offspring viability in the barn swallow.

Engagement of the external domains of CD45 tyrosine phosphatase can regulate the differentiation of immature CD4+CD8+ thymocytes into mature T cells.

Early discordant binocular vision disrupts signal transfer in the lateral geniculate nucleus.

Two highly homologous members of the ClC chloride channel family in both rat and human kidney.

Distinctive DNA conformation with enlarged major groove is found in Zn-finger-DNA and other protein-DNA complexes.

Loss of heterozygosity in cervical carcinoma: subchromosomal localization of a putative tumor-suppressor gene to chromosome 11q22-q24.

Relief of opsin desensitization and prolonged excitation of rod photoreceptors by 9-desmethylretinal.

A scheme for sequencing large DNA molecules by identifying local nuclear-induced effects.

A kinase-negative epidermal growth factor receptor that retains the capacity to stimulate DNA synthesis.

Selection of DNA clones with enhancer sequences.

Renal epithelial cells rapidly bind and internalize calcium oxalate monohydrate crystals.

The nuclear localization signal of the matrix protein of human immunodeficiency virus type 1 allows the establishment of infection in macrophages and quiescent T lymphocytes.

Mapping of the ligand binding domain of the transforming growth factor beta receptor type III by deletion mutagenesis.

Cytochrome P-450 mediates tissue-damaging hydroxyl radical formation during reoxygenation of the kidney.

JIP60, a methyl jasmonate-induced ribosome-inactivating protein involved in plant stress reactions.

Cloning and sequencing of thiol-specific antioxidant from mammalian brain: alkyl hydroperoxide reductase and thiol-specific antioxidant define a large family of antioxidant enzymes.

Dimerization of thiol-specific antioxidant and the essential role of cysteine 47.

Sertoli cell differentiation on basement membrane is mediated by the c-fos protooncogene.

Calorie restriction delays spontaneous tumorigenesis in p53-knockout transgenic mice.

Memory consolidation and the medial temporal lobe: a simple network model.

Differentiation between binding sites for angiotensin II and nonpeptide antagonists on the angiotensin II type 1 receptors.

Acidic transcription factors alleviate nucleosome-mediated repression of DNA replication of bovine papillomavirus type 1.

Central location of the Mu strong gyrase binding site is obligatory for optimal rates of replicative transposition.

Platelet-derived growth factor alpha receptor gene expression: isolation and characterization of the promoter and upstream regulatory elements.

Cloning and characterization of a basic helix-loop-helix protein expressed in early mesoderm and the developing somites.

Cell-surface receptors for gibbon ape leukemia virus and amphotropic murine retrovirus are inducible sodium-dependent phosphate symporters.

Identification of a melanosomal matrix protein encoded by the murine si (silver) locus using 'organelle scanning'.

Spermatophore size as determinant of paternity in an arctiid moth (Utetheisa ornatrix).

Transfection and transient expression of chloramphenicol acetyltransferase gene in the protozoan parasite Entamoeba histolytica.

Transient transfection of the enteric parasite Entamoeba histolytica and expression of firefly luciferase.

Secreted beta-amyloid precursor protein stimulates mitogen-activated protein kinase and enhances tau phosphorylation.

Major changes in the expression of the mRNAs for cholinergic differentiation factor/leukemia inhibitory factor and its receptor after injury to adult peripheral nerves and ganglia.

Glycopeptide enkephalin analogues produce analgesia in mice: evidence for penetration of the blood-brain barrier.

Fine mapping of a replication origin of human DNA.

Modification of a hydrogen bond to a bacteriochlorophyll a molecule in the light-harvesting 1 antenna of Rhodobacter sphaeroides.

High-affinity urokinase receptor antagonists identified with bacteriophage peptide display.

Atypical regions in large genomic DNA sequences.

Hepatocyte growth factor and macrophage inflammatory protein 1 beta: structurally distinct cytokines that induce rapid cytoskeletal changes and subset-preferential migration in T cells.

Primary structure and expression of the dihydropteroate synthetase gene of Plasmodium falciparum.

Transmembrane topology of two kainate receptor subunits revealed by N-glycosylation.

Piebald lethal (sl) acts early to disrupt the development of neural crest-derived melanocytes.

Tyr-129 is important to the peptide ligand affinity and selectivity of human endothelin type A receptor.

Nuclear localization signals also mediate the outward movement of proteins from the nucleus.

Intensity of natural selection at the major histocompatibility complex loci.

Mutations in ras genes in cells cultured from mouse skin tumors induced by ultraviolet irradiation.

Hot spots of retinoic acid synthesis in the developing spinal cord.

A direct mechanism for sensing low oxygen levels by central neurons.

KAR3-encoded kinesin is a minus-end-directed motor that functions with centromere binding proteins (CBF3) on an in vitro yeast kinetochore.

A fully active catalytic domain of bovine aspartyl (asparaginyl) beta-hydroxylase expressed in Escherichia coli: characterization and evidence for the identification of an active-site region in vertebrate alpha-ketoglutarate-dependent dioxygenases.

A 32P-postlabeling method for detecting unstable N-7-substituted deoxyguanosine adducts in DNA.

Structural analysis of chromosomal rearrangements associated with the developmental mutations Ph, W19H, and Rw on mouse chromosome 5.

The mitochondrial environment is required for activity of the cholesterol side-chain cleavage enzyme, cytochrome P450scc.

The chicken beta/epsilon-globin enhancer directs autonomously regulated, high-level expression of the chicken epsilon-globin gene in transgenic mice.

The H1A histone variant is an in vivo repressor of oocyte-type 5S gene transcription in Xenopus laevis embryos.

Sequence divergence of the red and green visual pigments in great apes and humans.

The gene for a recessively inherited human childhood progressive epilepsy with mental retardation maps to the distal short arm of chromosome 8.

Physical association of JAK1 and JAK2 tyrosine kinases with the interleukin 2 receptor beta and gamma chains.

A molecular phylogeny of the marine red algae (Rhodophyta) based on the nuclear small-subunit rRNA gene.

A gene phylogeny of the red algae (Rhodophyta) based on plastid rbcL.

Retinoic acid is necessary for development of the ventral retina in zebrafish.

Progressive growth in immunodeficient mice and host cell recruitment by mouse endothelial cells transformed by polyoma middle-sized T antigen: implications for the pathogenesis of opportunistic vascular tumors.

Controlled expression of plastid transgenes in plants based on a nuclear DNA-encoded and plastid-targeted T7 RNA polymerase.

C/EBP-related protein 2 confers lipopolysaccharide-inducible expression of interleukin 6 and monocyte chemoattractant protein 1 to a lymphoblastic cell line.

The Vpr protein of human immunodeficiency virus type 1 influences nuclear localization of viral nucleic acids in nondividing host cells.

A stable complex between integrase and viral DNA ends mediates human immunodeficiency virus integration in vitro.

The bifunctional iron-responsive element binding protein/cytosolic aconitase: the role of active-site residues in ligand binding and regulation.

Effects of monovalent cations and divalent metal ions on Escherichia coli selenophosphate synthetase.

Kinetics of exchange processes in the adsorption of proteins on solid surfaces.

Protein-tyrosine-phosphatase SHPTP2 couples platelet-derived growth factor receptor beta to Ras.

Replicative intermediates of human papillomavirus type 11 in laryngeal papillomas: site of replication initiation and direction of replication.

The ORD1 gene encodes a transcription factor involved in oxygen regulation and is identical to IXR1, a gene that confers cisplatin sensitivity to Saccharomyces cerevisiae.

Differential expression and activation of a family of murine peroxisome proliferator-activated receptors.

Energy transfer analysis of Fos-Jun dimerization and DNA binding.

Targeted insertions of two exogenous collagen genes into both alleles of their endogenous loci in cultured human cells: the insertions are directed by relatively short fragments containing the promoters and the 5' ends of the genes.

Isolation and characterization of the gene encoding 2,3-oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae.

Cloning of four cyclins from maize indicates that higher plants have three structurally distinct groups of mitotic cyclins.

Year: 1994 Volume: 91 Issue: 14

Year: 1994 Volume: 91 Issue: 13

Year: 1994 Volume: 91 Issue: 12

Year: 1994 Volume: 91 Issue: 11

Year: 1994 Volume: 91 Issue: 10

Year: 1994 Volume: 91 Issue: 9

Year: 1994 Volume: 91 Issue: 8

Year: 1994 Volume: 91 Issue: 7

Year: 1994 Volume: 91 Issue: 6

Year: 1994 Volume: 91 Issue: 5

Year: 1994 Volume: 91 Issue: 4

Year: 1994 Volume: 91 Issue: 3

Year: 1994 Volume: 91 Issue: 2

Year: 1994 Volume: 91 Issue: 1

Impact melting of frozen oceans on the early Earth: Implications for the origin of life

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Evidence for alternating head catalysis by kinesin during microtubule-stimulated ATP hydrolysis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hackney, D. D.
Description	Author Affiliation: Hackney DD ( Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213.);
Abstract	The N-terminal 392 amino acids of the Drosophila kinesin alpha subunit (designated DKH392) form a dimer in solution that releases only one of its two tightly bound ADP molecules on association with a microtubule, whereas a shorter monomeric construct (designated DKH340) releases > or = 95% of its one bound ADP on association with a microtubule. This half-site reactivity of dimeric DKH392 is observed over a wide range of ratios of DKH392 to microtubules and steady-state ATPase rates, indicating that it is characteristic of the mechanism of microtubule-stimulated ATP hydrolysis and not the result of a fortuitous balance of rate constants. When [alpha-32P]ATP is included in the medium, incorporation of 32P label into the pool of ADP that is bound to the complex of DKH392 and microtubules occurs rapidly enough for the bound ADP to be an intermediate on the main pathway of ATP hydrolysis. These and other results are consistent with the half-site reactivity being a consequence of the tethering of dimeric DKH392 to the microtubule through one head domain, which is attached in a rigor-like manner without bound nucleotide, whereas the other head is not attached to the microtubule and still contains a tightly bound ADP. An intermediate of this nature and the tight binding of DKH392 to microtubules in the presence of ATP suggest a mechanism for directed motility in which the head domains of dimeric DKH392 alternate in a sequential manner.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	15
Volume Number	91
Language	English
Publisher	National Academy of Sciences
Publisher Date	1994-08-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Adenosine Triphosphate Metabolism Kinesin Microtubules Animals Catalysis Drosophila Hydrolysis Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in