NDLI: Hexokinase II in CD133+ and CD133- hepatoma BEL-7402 Cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Gong, Lei Cui, Zhuqingqing Yu, Xin Wei, Yuhua Peng, Jirun Leng, Xisheng
Description	Author Affiliation: Gong L ( Department of Hepatobiliary Surgery, Peking University People's Hospital, No. 11 South Xizhimen Street, Xicheng District, Beijing, 100044, People's Republic of China.)
Abstract	Hexokinase II is a key enzyme in the glycolytic pathway and CD133+ human hepatoma cells possess cancer stem cell-like properties. The expression and enzyme activity of hexokinase II in CD133+ and CD133- hepatoma cells were examined. CD133 on the surface of the hepatoma BEL-7402 cells was analyzed by flow cytometry and the cells were magnetically sorted into CD133+ and CD133- groups. CD133+ cells comprised 1.04% of the total BEL-7402 cell population. Reverse transcription-polymerase chain reaction (PCR) and quantitative real-time PCR were used to assay the expression of hexokinase II mRNA in these two groups. The level of mRNA in CD133- cells was 4.35 times greater than the level in CD133+ cells. 3,6-biphosphoglucose dehydrogenase-coupled colorimetric method and temperature-sensitive trials were applied to determine the enzyme activity of hexokinase II, which was 1.02 U/g protein in CD133+ cells and 2.47 U/g protein in CD133- cells. Hexokinase II was the major active hexokinase isoform in CD133+ cells, comprising 92.7% of the overall cellular hexokinase activity. The results indicate that hexokinase II is vitally meaningful for CD133+ hepatoma BEL-7402 cells. Hexokinase II represents a new therapeutic target for treating CD133+ hepatoma cells.
File Format	HTM / HTML
ISSN	12194956
Issue Number	2
Volume Number	18
e-ISSN	15322807
Journal	Pathology & Oncology Research
Language	English
Publisher	Springer
Publisher Date	2012-04-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Oncology Antigens, Cd Metabolism Carcinoma, Hepatocellular Glycoproteins Hexokinase Liver Neoplasms Neoplastic Stem Cells Peptides Genetics Cell Line, Tumor Flow Cytometry Humans Immunoenzyme Techniques Rna, Messenger Real-time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Journal Article
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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