NDLI: Targeting MET by tyrosine kinase inhibitor suppresses growth and invasion of nasopharyngeal carcinoma cell lines.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lau, Patrick C. Wong, Elaine Y.
Description	Country affiliation: China Author Affiliation: Lau PC ( State Key Laboratory of Oncology in South China, Sir YK Pao Center for Cancer, Department of Clinical Oncology, Hong Kong Cancer Institute, Shatin, Hong Kong SAR, China. patricklau75@hotmail.com)
Abstract	Nasopharyngeal carcinoma (NPC) represents a common cancer in endemic areas with high invasive and metastatic potential. It is now known that the HGF-MET signaling pathway plays an important role in mediating the invasive growth of many different types of cancer, including head and neck squamous cell carcinoma. HGF has been shown to stimulate NPC cell growth and invasion in cell line model. The current study aims at demonstrating the effect of MET inhibition by small molecule tyrosine kinase inhibitor PHA665752 on the growth and invasive potential of NPC cell lines. NPC cell lines were used for immunohistochemistry for the MET protein, as well as western blot analysis on MET together with its downstream cascade signaling proteins after treatment with PHA665752. The effect on cell growth, migration and invasion after PHA665752 treatment was also studied. MET inhibition by PHA665752 resulted in highly significant inhibition on NPC cell growth, migration and invasion in vitro. Down-regulation of phospho-MET, phospho-Akt, phospho-MAPK, phospho-STAT3, cyclin D1, ß-catenin and PCNA was detected in NPC cells after PHA665752 treatment. MET inhibition with tyrosine kinase inhibitor resulted in suppression of NPC cell growth and invasive potential via down-regulation of a variety of signaling onco-proteins. MET is an important therapeutic target for NPC that warrants further studies and clinical trials.
File Format	HTM / HTML
ISSN	12194956
Issue Number	2
Volume Number	18
e-ISSN	15322807
Journal	Pathology & Oncology Research
Language	English
Publisher	Springer
Publisher Date	2012-04-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Oncology Cell Movement Drug Effects Cell Proliferation Indoles Pharmacology Nasopharyngeal Neoplasms Drug Therapy Pathology Proto-oncogene Proteins C-met Antagonists & Inhibitors Metabolism Sulfones Apoptosis Blotting, Western Carcinoma, Squamous Cell Cell Adhesion Humans Immunoenzyme Techniques Neoplasm Invasiveness Tumor Cells, Cultured Journal Article
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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