NDLI: Quantitation of plasma circulating DNA using quantitative PCR for the detection of hepatocellular carcinoma.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Huang, Zhaohui Hua, Dong Hu, Yu Cheng, Zhihong Zhou, Xike Xie, Qigen Wang, Qiongyao Wang, Feng Du, Xiang Zeng, Yanjun
Description	Country affiliation: China Author Affiliation: Huang Z ( Wuxi Oncology Institute, The Fourth Affiliated Hospital of Suzhou University, 200 Huihe Road, Wuxi, 214062, Jiangsu Province, China. hzhwxsy@126.com)
Abstract	Circulating DNA is a potential biomarker for tumor diagnosis and prognosis. This study was aimed to quantify the circulating DNA in plasma from patients with hepatocellular carcinoma (HCC) using quantitative PCR and evaluate its potential clinical value. Blood samples were collected from 72 patients with HCC, 37 with liver cirrhosis or chronic hepatitis and 41 healthy volunteers. Plasma DNA was extracted and quantified by a real-time quantitative PCR method. The diagnostic and prognostic value of plasma DNA analysis for HCC was evaluated. DNA levels in the HCC plasma (median: 173 ng/mL) were significantly higher than those in the healthy controls (9 ng/mL) or control benign patients (46 ng/mL) (P < 0.001). The area under the receiver-operation characteristic (ROC) curve (AUC) assessing plasma DNA was 0.949 for healthy controls and 0.874 for control patients. Plasma DNA detection could discriminate HCC from normal controls with 90.2% sensitivity and 90.3% specificity at the cut-off value of 18.2 ng/mL. Combined ROC analyses using plasma DNA and serum AFP revealed an elevated AUC of 0.974 with 95.1% sensitivity and 94.4% specificity in discriminating HCC from normal controls. The plasma DNA levels were positively associated with tumor size (P = 0.012), and were significantly elevated in HCC patients with intrahepatic spreading or vascular invasion (P = 0.035). The overall survival time of patients with high plasma DNA levels showed a shortened tread when compared with that of patients with low plasma DNA concentrations (P = 0.071). Plasma DNA may be a valuable noninvasive tool for the detecting and predicting the metastasis potential of HCC; and the prognostic value of plasma DNA needed further investigation.
File Format	HTM / HTML
ISSN	12194956
Issue Number	2
Volume Number	18
e-ISSN	15322807
Journal	Pathology & Oncology Research
Language	English
Publisher	Springer
Publisher Date	2012-04-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Oncology Tumor Markers, Biological Blood Carcinoma, Hepatocellular Diagnosis Dna Hepatitis, Chronic Liver Cirrhosis Liver Neoplasms Genetics Mortality Case-control Studies Female Humans Male Middle Aged Neoplasm Staging Prognosis Roc Curve Real-time Polymerase Chain Reaction Survival Rate Comparative Study Journal Article
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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