| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Sohn, Sung-Hwa Lee, Ji Min Park, Soojin Yoo, Hyun Kang, Jeong Wook Shin, Dasom Jung, Kyung-Hwa Lee, Yun-Sil Cho, Jaeho Bae, Hyunsu |
| Description |
Country affiliation: South Korea Author Affiliation: Sohn SH ( Department of Radiation Oncology, Yonsei University College of Medicine, Seoul, South Korea.); Lee JM ( Department of Radiation Oncology, Yonsei University College of Medicine, Seoul, South Korea.); Park S ( Department of Physiology, College of Oriental Medicine, Kyung Hee University, Seoul, South Korea.); Yoo H ( Department of Radiation Oncology, Yonsei University College of Medicine, Seoul, South Korea.); Kang JW ( Department of Radiation Oncology, Yonsei University College of Medicine, Seoul, South Korea.); Shin D ( Department of Physiology, College of Oriental Medicine, Kyung Hee University, Seoul, South Korea.); Jung KH ( Department of Physiology, College of Oriental Medicine, Kyung Hee University, Seoul, South Korea.); Lee YS ( College of Pharmacy & Division of Life and Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, South Korea.); Cho J ( Department of Radiation Oncology, Yonsei University College of Medicine, Seoul, South Korea. Electronic address: jjhmd@yuhs.ac.); Bae H ( Department of Physiology, College of Oriental Medicine, Kyung Hee University, Seoul, South Korea. Electronic address: hbae@khu.ac.kr.) |
| Abstract |
Although lung inflammation and fibrosis are well-documented dose-limiting side effects of lung irradiation, the mechanisms underlying these pathologies are unknown. An improved mechanistic understanding of radiation-induced pneumonitis is a prerequisite for the development of more effective radiotherapy; this was the rationale for the current study. Mouse lungs were focally irradiated with 75 Gy. The numbers of neutrophils, lymphocytes, macrophages, and total cells in the bronchoalveolar lavage fluid were counted, and pro-inflammatory cytokine levels were measured. Histological analysis and immunohistochemical staining for Tgf-ß1 and Cd68 (a macrophage-specific protein) was also performed. After irradiation, mice developed pneumonitis, and exhibited higher numbers of neutrophils, lymphocytes, eosinophils, macrophages, and total cells compared to controls. In addition, inflammasome (Nlrp3, and caspase 1, Il1a, and Il1ß), adhesion molecule (Vcam1), and cytokine (Il6) genes were significantly upregulated in the IR group. Cd68 and Tgfb1 proteins were significantly increased after irradiation. Upregulation of Cd68 and Tgfb1 correlates with the onset of radiation-induced pneumonitis and fibrosis. In addition, radiation-induced pneumonitis and fibrosis are accompanied by upregulation of phenotypic markers of inflammasome activity. Our findings have implications for the onset and exacerbation of damage in normal lung tissue. |
| File Format |
HTM / HTML |
| ISSN |
13826689 |
| Issue Number |
2 |
| Volume Number |
39 |
| e-ISSN |
18727077 |
| Journal |
Environmental Toxicology and Pharmacology |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2015-03-01 |
| Publisher Place |
Netherlands |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Environmental Health Discipline Pharmacology Carrier Proteins Genetics Inflammasomes Pneumonia Immunology Radiation Injuries, Experimental Animals Antigens, Cd Antigens, Differentiation, Myelomonocytic Bronchoalveolar Lavage Fluid Cytology Caspase 1 Cell Count Cytokines Female Fibrosis Lung Pathology Mice, Inbred C57bl Vascular Cell Adhesion Molecule-1 Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Health, Toxicology and Mutagenesis Medicine Toxicology Pharmacology |
