NDLI: A topoisomerase II poison screen of ethnomedicinal Thai plants using a yeast cell-based assay.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sangmalee, Suphattra Laorpaksa, Areerat Sukrong, Suchada
Spatial Coverage	Thailand
Description	Country affiliation: Thailand Author Affiliation: Sangmalee S ( Department of Pharmacognosy and Pharmaceutical Botany, CU-Drug & Health Product Innovation Center, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.)
Abstract	ETHNOPHARMACOLOGICAL RELEVANCE: The plants used in this study had previously been identified and used by Thai folk practitioners, who had knowledge of Thai traditional medicine, as alternative treatments for cancer. Investigation into the mechanism of the Topoisomerase II (Top2) poison of these plants may give rise to new drug leads for cancer treatment. AIM OF THE STUDY: This study aimed to screen ethnomedicinal plants used in Thai traditional medicine for Top2 poison activity using a yeast cell-based assay and also to validate the traditional uses of these plants by examining the Top2 poison activity. MATERIALS AND METHODS: Thirty Thai medicinal plants were harvested and identified. Plant methanol extracts were prepared and screened in vitro using a yeast cell-based assay. Mutant yeast strains carrying the top2-1 allele, which encodes a temperature-sensitive topoisomerase, were used to establish the yeast spot test. Strains carrying this mutation grow normally at 25°C and generally have a wild-type drug sensitivity. These yeast strains are able to grow at 30°C, but the Top2 activity is greatly reduced at this temperature, which causes the strains to be highly drug resistant to anti-Top2 agents. Cell growth was measured by colony survival after exposure to various concentrations of extracts at different temperatures. RESULTS: The extracts of six out of thirty ethnomedicinal plants, Curcuma longa, C. zedoaria, Derris scandens, Grangea maderaspatana, Stephania pierrei and S. suberosa, were found to have Top2 poison activity against the yeast cells. CONCLUSIONS: The yeast screening system confirmed the proposed anti-cancer mechanisms of plants used in Thai traditional medicine by traditional doctors.
File Format	HTM / HTML
ISSN	03788741
Issue Number	2
Volume Number	142
e-ISSN	18727573
Journal	Journal of Ethnopharmacology
Language	English
Publisher	Elsevier
Publisher Date	2012-07-13
Publisher Place	Ireland
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Ethnopharmacology Angiosperms Antineoplastic Agents, Phytogenic Pharmacology Dna Topoisomerases, Type Ii Metabolism Plant Extracts Plants, Medicinal Topoisomerase Ii Inhibitors Yeasts Drug Effects Alleles Biological Assay Methods Curcuma Derris Drug Resistance Medicine, Traditional Mutation Phytotherapy Stephania Temperature Thailand Genetics Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Drug Discovery Pharmacology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Anti-proliferative and apoptosis induction activities of extracts from Thai medicinal plant recipes selected from MANOSROI II database.

Screening of plants used in Mayan traditional medicine to treat cancer-like symptoms.

In vitro anti-proliferative activity on colon cancer cell line (HT-29) of Thai medicinal plants selected from Thai/Lanna medicinal plant recipe database 'MANOSROI III'.

Traditionally used Thai medicinal plants: in vitro anti-inflammatory, anticancer and antioxidant activities.

Ethnomedicinal and ethnopharmaco-statistical studies of Eastern Rajasthan, India.

Review on ethnomedicinal, phytochemical and pharmacological evidence of Himalayan anticancer plants.

Antiplasmodial and cytotoxic activities of medicinal plants traditionally used in the village of Kiohima, Uganda.

Anticancer and multidrug-resistance reversing potential of traditional medicinal plants and their bioactive compounds in leukemia cell lines.

Hypoglycemic activity of Thai medicinal plants selected from the Thai/Lanna Medicinal Recipe Database MANOSROI II.

A topoisomerase II poison screen of ethnomedicinal Thai plants using a yeast cell-based assay.

Similar Documents

Anti-proliferative and apoptosis induction activities of extracts from Thai medicinal plant recipes selected from MANOSROI II database.

Screening of plants used in Mayan traditional medicine to treat cancer-like symptoms.

In vitro anti-proliferative activity on colon cancer cell line (HT-29) of Thai medicinal plants selected from Thai/Lanna medicinal plant recipe database 'MANOSROI III'.

Traditionally used Thai medicinal plants: in vitro anti-inflammatory, anticancer and antioxidant activities.

Ethnomedicinal and ethnopharmaco-statistical studies of Eastern Rajasthan, India.

Review on ethnomedicinal, phytochemical and pharmacological evidence of Himalayan anticancer plants.

Antiplasmodial and cytotoxic activities of medicinal plants traditionally used in the village of Kiohima, Uganda.

Anticancer and multidrug-resistance reversing potential of traditional medicinal plants and their bioactive compounds in leukemia cell lines.

Hypoglycemic activity of Thai medicinal plants selected from the Thai/Lanna Medicinal Recipe Database MANOSROI II.

A topoisomerase II poison screen of ethnomedicinal Thai plants using a yeast cell-based assay.