NDLI: Traditionally used Thai medicinal plants: in vitro anti-inflammatory, anticancer and antioxidant activities.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Siriwatanametanon, Nisarat Fiebich, Bernd L. Efferth, Thomas Prieto, Jose M. Heinrich, Michael
Spatial Coverage	Thailand
Description	Country affiliation: United kingdom Author Affiliation: Siriwatanametanon N ( Centre for Pharmacognosy and Phytotherapy, The School of Pharmacy, University of London, London, United Kingdom.)
Abstract	AIMS OF THE STUDY: In order to assess traditional Thai claims about the therapeutic potential of medicinal plants and to select plants for future phytochemical research, nine plant species with anti-inflammatory uses were selected from Thai textbooks and assessed for their in vitro anti-inflammatory, antiproliferative and antioxidant activities. METHODS: Nuclear factor-kappaB (NF-kappaB) inhibitory effects in stably transfected HeLa cells were determined by luciferase assay, and effects on LPS-induced pro-inflammatory mediators prostaglandin E2 (PGE2), interleukin (IL)-6, IL-1beta, and tumour necrosis factor (TNF)alpha in primary monocytes were assessed by ELISA. Cytotoxic activities were examined against HeLa cells, human leukaemia CCRF-CEM cells and the multidrug-resistant CEM/ADR5000 subline using the MTT and XTT tests. However, a redox status has been linked with both inflammation and cancer, antioxidant effects were also assessed using the DPPH, lipid-peroxidation, and Folin-Ciocalteau methods. RESULTS: Among all the nine species, Gynura pseudochina var. hispida and Oroxylum indicum showed the most promising NF-kappaB inhibitory effects with the lowest IC(50) values (41.96 and 47.45 microg/ml, respectively). Muehlenbeckia platyclada did not inhibit the NF-kappaB activation but effectively inhibited the release of IL-6, IL-1beta and TNF-alpha with IC(50) values ranging between 0.28 and 8.67 microg/ml. Pouzolzia indica was the most cytotoxic against CCRF-CEM cells and the multidrug-resistant CEM/ADR5000 cells (9.75% and 10.48% viability, at 10 microg/ml, respectively). Rhinacanthus nasutus was the most potent cytotoxicity against HeLa cells (IC(50) 3.63 microg/ml) and showed specific cytotoxicity against the multidrug-resistant CEM/ADR5000 cells (18.72% viability at 10 microg/ml, p<0.0001 when compared to its cytotoxicity against CCRF-CEM cells). Moreover, Oroxylum indicum showed a high level of antioxidant activity by inhibiting lipid-peroxidation (IC(50) 0.08 microg/ml). CONCLUSIONS: This study provides in vitro evidence for the use of the Thai plants, most importantly Gynura pseudochina var. hispida, Oroxylum indicum and Muehlenbeckia platyclada as Thai anti-inflammatory remedies and these plants are now a priority for further phytochemical research.
File Format	HTM / HTML
ISSN	03788741
Issue Number	2
Volume Number	130
Journal	Journal of Ethnopharmacology
e-ISSN	18727573
Language	English
Publisher	Elsevier
Publisher Date	2010-07-20
Publisher Place	Ireland
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Ethnopharmacology Angiosperms Anti-inflammatory Agents Pharmacology Antineoplastic Agents, Phytogenic Antioxidants Plant Extracts Asteraceae Bignoniaceae Cell Survival Drug Effects Dinoprostone Metabolism Dose-response Relationship, Drug Drug Resistance, Neoplasm Hela Cells Humans Inflammation Mediators Inhibitory Concentration 50 Interleukin-1beta Interleukin-6 Genetics Lipid Peroxidation Medicine, Traditional Monocytes Immunology Nf-kappa B Antagonists & Inhibitors Phenols Analysis Chemistry Plants, Medicinal Polygonaceae Thailand Transfection Tumor Necrosis Factor-alpha Comparative Study Journal Article
Content Type	Text
Resource Type	Article
Subject	Drug Discovery Pharmacology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

DXXK exerts anti-inflammatory effects by inhibiting the lipopolysaccharide-induced NF-κB/COX-2 signalling pathway and the expression of inflammatory mediators.

Effects of taraxasterol on inflammatory responses in lipopolysaccharide-induced RAW 264.7 macrophages.

In vitro anti-proliferative activity on colon cancer cell line (HT-29) of Thai medicinal plants selected from Thai/Lanna medicinal plant recipe database 'MANOSROI III'.

Anti-proliferative activities on HeLa cancer cell line of Thai medicinal plant recipes selected from MANOSROI II database.

Potent anti-cervical cancer activity: synergistic effects of Thai medicinal plants in recipe N040 selected from the MANOSROI III database.

Anti-inflammatory effects of Polygala tenuifolia root through inhibition of NF-κB activation in lipopolysaccharide-induced BV2 microglial cells.

Anti-proliferative and apoptosis induction activities of extracts from Thai medicinal plant recipes selected from MANOSROI II database.

A topoisomerase II poison screen of ethnomedicinal Thai plants using a yeast cell-based assay.

Topical anti-inflammatory and analgesic activity of kirenol isolated from Siegesbeckia orientalis.

Traditionally used Thai medicinal plants: in vitro anti-inflammatory, anticancer and antioxidant activities.

Similar Documents

DXXK exerts anti-inflammatory effects by inhibiting the lipopolysaccharide-induced NF-κB/COX-2 signalling pathway and the expression of inflammatory mediators.

Effects of taraxasterol on inflammatory responses in lipopolysaccharide-induced RAW 264.7 macrophages.

In vitro anti-proliferative activity on colon cancer cell line (HT-29) of Thai medicinal plants selected from Thai/Lanna medicinal plant recipe database 'MANOSROI III'.

Anti-proliferative activities on HeLa cancer cell line of Thai medicinal plant recipes selected from MANOSROI II database.

Potent anti-cervical cancer activity: synergistic effects of Thai medicinal plants in recipe N040 selected from the MANOSROI III database.

Anti-inflammatory effects of Polygala tenuifolia root through inhibition of NF-κB activation in lipopolysaccharide-induced BV2 microglial cells.

Anti-proliferative and apoptosis induction activities of extracts from Thai medicinal plant recipes selected from MANOSROI II database.

A topoisomerase II poison screen of ethnomedicinal Thai plants using a yeast cell-based assay.

Topical anti-inflammatory and analgesic activity of kirenol isolated from Siegesbeckia orientalis.

Traditionally used Thai medicinal plants: in vitro anti-inflammatory, anticancer and antioxidant activities.