NDLI: Total mercury determination in different tissues of broiler chicken by using cloud point extraction and cold vapor atomic absorption spectrometry.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Shah, A. Q. Kazi, T. G. Baig, J. A. Afridi, H. I. Kandhro, G. A. Arain, M. B. Kolachi, N. F. Wadhwa, S. K.
Spatial Coverage	Pakistan
Description	Country affiliation: Pakistan Author Affiliation: Shah AQ ( Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan. aqshah07@yahoo.com)
Abstract	A cloud point extraction (CPE) method has been developed for the determination of total mercury (Hg) in different tissues of broiler chicken by cold vapor atomic absorption spectrometry (CVAAS). The broiler chicken tissues (leg, breast, liver and heart) were subjected to microwave assisted digestion in a mixture of nitric acid and hydrogen peroxide (2:1 ratio), prior to preconcentration by CPE. Various parameters such as the amount of ammonium O,O-diethyldithiophosphate (DDTP), concentrations of Triton X-114, equilibrium temperature, time and centrifugation have been studied in order to find the best conditions for the determination of mercury. For validation of proposed method a certified reference material, DORM-2 was used. No significant difference p>0.05 was observed between the experimental results and the certified values of CRM (paired t-test). The limit of detection and quantitation obtained under the optimal conditions were 0.117 and 0.382 microg/kg, respectively. The accumulation of Hg in different tissues were found in the order of, liver>muscles>heart. The concentration of Hg in chicken tissues were found in the range of 1.57-2.75, 1.40-2.27, 1.55-4.22, and 1.39-2.61 microg/kg in leg, breast, liver and heart, respectively.
File Format	HTM / HTML
ISSN	02786915
Issue Number	1
Volume Number	48
e-ISSN	18736351
Journal	Food and Chemical Toxicology
Language	English
Publisher	Elsevier
Publisher Date	2010-01-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Toxicology__semicolon__nutritional Discipline Sciences Chickens Metabolism Mercury Analysis Animals Calibration Indicators And Reagents Liver Chemistry Muscle, Skeletal Myocardium Pakistan Reference Values Spectrophotometry, Atomic Surface-active Agents Temperature Journal Article
Content Type	Text
Resource Type	Article
Subject	Medicine Toxicology Food Science

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