NDLI: Tissue-Engineered Tracheal Reconstruction Using Mesenchymal Stem Cells Seeded on a Porcine Cartilage Powder Scaffold

Content Provider	Springer Nature Link
Author	Shin, Yoo Seob Choi, Jae Won Park, Ju Kyeong Kim, Yoo Suk Yang, Soon Sim Min, Byoung Hyun Kim, Chul Ho
Copyright Year	2014
Abstract	Tissue engineering using a biocompatible scaffold with various cells might be a solution for tracheal reconstruction. We investigated the plausibility of using mesenchymal stem cells (MSCs) seeded on a porcine cartilage powder (PCP) scaffold for tracheal defect repair. PCP made with minced and decellularized porcine articular cartilage was molded into a 5 × 12 mm (height × diameter) scaffold. MSCs from young rabbit bone marrow were expanded and cultured with the PCP scaffold. After 7 weeks culture, the tracheal implants were transplanted on a 5 × 10 mm tracheal defect in six rabbits. 6 and 10 weeks postoperatively, the implanted area was evaluated. None of the six rabbits showed any sign of respiratory distress. Endoscopic examination revealed that respiratory epithelium completely covered the regenerated trachea and there were no signs of collapse or blockage. A patent luminal contour of the trachea was observed on the computed tomography scan in all six rabbits and the reconstructed areas were not narrow compared to normal adjacent trachea. Histologic examination showed that neo-cartilage was successfully produced with minimal inflammation or granulation tissue. Ciliary beating frequency of the regenerated epithelium was not significantly different from the normal adjacent mucosa. MSCs cultured with a PCP scaffold successfully restored not only the shape but also the function of the trachea without any graft rejection.
Starting Page	1003
Ending Page	1013
Page Count	11
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	43
Issue Number	4
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2014-09-25
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Tracheal regeneration Porcine cartilage powder Tissue engineering Mesenchymal stem cell Biomedicine general Biomedical Engineering Biophysics and Biological Physics Mechanics Biochemistry
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Evaluation of Cartilage Repair by Mesenchymal Stem Cells Seeded on a PEOT/PBT Scaffold in an Osteochondral Defect

Chondrocytes, Mesenchymal Stem Cells, and Their Combination in Articular Cartilage Regenerative Medicine

In vivo MR Imaging of Tissue-engineered Human Mesenchymal Stem Cells Transplanted to Mouse: a Preliminary Study

High-Throughput Screening for Modulators of Mesenchymal Stem Cell Chondrogenesis

Non-Invasive Time-Lapsed Monitoring and Quantification of Engineered Bone-Like Tissue

Muscle Tissue Engineering Using Gingival Mesenchymal Stem Cells Encapsulated in Alginate Hydrogels Containing Multiple Growth Factors

Isolation, Characterization, and Differentiation of Stem Cells for Cartilage Regeneration

Tracheal cartilage regeneration by progenitor cells derived from the perichondrium

High Field Sodium MRI Assessment of Stem Cell Chondrogenesis in a Tissue-Engineered Matrix

Tissue-Engineered Tracheal Reconstruction Using Mesenchymal Stem Cells Seeded on a Porcine Cartilage Powder Scaffold

Similar Documents

Evaluation of Cartilage Repair by Mesenchymal Stem Cells Seeded on a PEOT/PBT Scaffold in an Osteochondral Defect

Chondrocytes, Mesenchymal Stem Cells, and Their Combination in Articular Cartilage Regenerative Medicine

In vivo MR Imaging of Tissue-engineered Human Mesenchymal Stem Cells Transplanted to Mouse: a Preliminary Study

High-Throughput Screening for Modulators of Mesenchymal Stem Cell Chondrogenesis

Non-Invasive Time-Lapsed Monitoring and Quantification of Engineered Bone-Like Tissue

Muscle Tissue Engineering Using Gingival Mesenchymal Stem Cells Encapsulated in Alginate Hydrogels Containing Multiple Growth Factors

Isolation, Characterization, and Differentiation of Stem Cells for Cartilage Regeneration

Tracheal cartilage regeneration by progenitor cells derived from the perichondrium

High Field Sodium MRI Assessment of Stem Cell Chondrogenesis in a Tissue-Engineered Matrix

Tissue-Engineered Tracheal Reconstruction Using Mesenchymal Stem Cells Seeded on a Porcine Cartilage Powder Scaffold