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Tracheal cartilage regeneration by progenitor cells derived from the perichondrium

Tissue Engineering and Regenerative Medicine : Volume 10, Issue 4, August 2013

Tissue Engineering and Regenerative Medicine : Volume 10, Issue 3, June 2013

Tissue Engineering and Regenerative Medicine : Volume 10, Issue 2, April 2013

Tissue Engineering and Regenerative Medicine : Volume 10, Issue 1, February 2013

Tissue Engineering and Regenerative Medicine : Volume 9

Tracheal cartilage regeneration by progenitor cells derived from the perichondrium

Content Provider	Springer Nature Link
Author	Jang, Tae Won Yoon, Myung Hee Kim, Ji Hae Lee, Sang Joon Heo, Jeong Hoon Chun, Bong Kwon Jung, Maan Hong Oak, Chul Ho
Copyright Year	2013
Abstract	The regenerative potential of adult tracheal cartilage is very limited which makes repair of this cartilage extremely challenging. However, the perichondrium has a large quantity of progenitor cells that differentiate directly into chondrocytes. Bone morphogenetic protein (BMP) stimulates chondrogenesis by inducing the differentiation of progenitor cells into chondrocytes which makes it a potential therapeutic agent for cartilage regeneration. In this present study, we investigated the mesenchymal stem cell characterization of progenitor cells from the perichondrial tissue of tracheal cartilage and the efficiency of the perichondrium for tracheal cartilage regeneration by evaluating the effect of BMP on cartilage regeneration in perichondrial progenitor cells. Progenitor cells were isolated from the fibrous perichodrial tissue of tracheal cartilage and cultured for phenotypic characterization of mesenchymal stem cells. Next, a 0.3–0.5 mm gap was made in the mid-ventral portion of the tracheal cartilages. In the control group (n=5), the resulting gap was left untreated (without perichondrial replacement). In the perichondrial group (n=15), the gap was replaced by fibrous perichondrial tissue. We euthanized all mice in each group (n=5) at 2, 4, and 20 weeks after the perichondrial replacement and examined the replacement site microscopically. The immunohistochemistry for BMP-2 was performed in progenitor cells from perichondrial tissue. The primary cultured cells from fibrous perichondrial tissue were positive for CD29, CD90, and CD105, but negative for CD45. The progenitor cells were capable of multipotent differentiation towards adipogenic and osteogenic lineages. The control group showed no regenerated cartilage 2 weeks after injury. The experimental group demonstrated proliferation of progenitor cells between both stumps at 2 weeks and regenerated hyaline cartilage was observed at 4 and 20 weeks. Regenerated cartilage was observed as separated islets between the ends of the host cartilage stumps. The gaps were nearly filled with regenerated cartilage at 20 weeks. BMP-2 was expressed in both fibrous and cabial perichondria chondrogenesis. The progenitor cells from perichondrial tissue had mesenchymal stem cell-like features and might play a crucial role in tracheal cartilage regeneration and repair. Expression of BMP-2 may induce formation of structural cartilage in a tracheal injury model.
Starting Page	286
Ending Page	292
Page Count	7
File Format	PDF
ISSN	17382696
Journal	Tissue Engineering and Regenerative Medicine
Volume Number	10
Issue Number	5
e-ISSN	22125469
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-08-22
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biomedical Engineering mesenchymal stem cell perichondrium tracheal cartilage chondrogenesis progenitor cell Biomedicine general Cell Biology
Content Type	Text
Resource Type	Article
Subject	Medicine Biomedical Engineering

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