NDLI: Mechanical Stimulation of Bone Marrow In Situ Induces Bone Formation in Trabecular Explants

Content Provider	Springer Nature Link
Author	Birmingham, E. Kreipke, T. C. Dolan, E. B. Coughlin, T. R. Owens, P. McNamara, L. M. Niebur, G. L. McHugh, P. E.
Copyright Year	2014
Abstract	Low magnitude high frequency (LMHF) loading has been shown to have an anabolic effect on trabecular bone in vivo. However, the precise mechanical signal imposed on the bone marrow cells by LMHF loading, which induces a cellular response, remains unclear. This study investigates the influence of LMHF loading, applied using a custom designed bioreactor, on bone adaptation in an explanted trabecular bone model, which isolated the bone and marrow. Bone adaptation was investigated by performing micro CT scans pre and post experimental LMHF loading, using image registration techniques. Computational fluids dynamic models were generated using the pre-experiment scans to characterise the mechanical stimuli imposed by the loading regime prior to adaptation. Results here demonstrate a significant increase in bone formation in the LMHF loaded group compared to static controls and media flow groups. The calculated shear stress in the marrow was between 0.575 and 0.7 Pa, which is within the range of stimuli known to induce osteogenesis by bone marrow mesenchymal stem cells in vitro. Interestingly, a correlation was found between the bone formation balance (bone formation/resorption), trabecular number, trabecular spacing, mineral resorption rate, bone resorption rate and mean shear stresses. The results of this study suggest that the magnitude of the shear stresses generated due to LMHF loading in the explanted bone cores has a contributory role in the formation of trabecular bone and improvement in bone architecture parameters.
Starting Page	1036
Ending Page	1050
Page Count	15
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	43
Issue Number	4
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2014-10-04
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Trabecular bone Bone marrow Shear stress Low magnitude high frequency loading Vibration Mechanobiology Biomedicine general Biomedical Engineering Biophysics and Biological Physics Mechanics Biochemistry
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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