NDLI: In vivo MR Imaging of Tissue-engineered Human Mesenchymal Stem Cells Transplanted to Mouse: a Preliminary Study

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Annals of Biomedical Engineering : Volume 34

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In vivo MR Imaging of Tissue-engineered Human Mesenchymal Stem Cells Transplanted to Mouse: a Preliminary Study

Content Provider	Springer Nature Link
Author	Ko, In Kap Song, Ho Taek Cho, Eun Jin Lee, Eun Sook Huh, Yong Min Suh, Jin Suck
Copyright Year	2006
Abstract	Current progress integrating stem cell biology and tissue engineering techniques has been invaluable to clinical applications. Prior to the application of celluar transplantation technique to patients, we need to establish techniques that can monitor their tissue biodistribution non-invasively. In this study, we proposed an imaging modality using MRI to not only monitor implanted scaffold in vivo, but also to track transplanted cells and behavior around the implant. For this purpose, human bone marrow-derived mesenchymal stem cells (hMSCs) were labeled with superparamagnetic iron oxide (Feridex) and then labeled hMSCs were cultured in a gelatin sponge used as a scaffold to support cell growth and proliferation. Histological assessment and MTT assay showed that cell labeling with MR contrast agent did not harm cell viability. Also, Feridex-labeled hMSCs showed a significant decrease in T2 signal intensity, even within the gelatin sponge in vitro. After implanting the sponge/cell complex in vivo, we could visualize cellular behavior around the implant over time using a noninvasive MRI modality and this finding was correlated with histological study, which illustrates the potential of a new approach proposed here for in vivo monitoring of implanted cell-based tissue-engineered product.
Starting Page	101
Ending Page	108
Page Count	8
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	35
Issue Number	1
e-ISSN	15739686
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2006-11-03
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	MRI (magnetic resonance imaging) Scaffold Mesenchymal stem cell Transplantation Cell migration Biochemistry Mechanics Biophysics/Biomedical Physics Biomedical Engineering Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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