NDLI: Isothermal DNA origami folding: avoiding denaturing conditions for one-pot, hybrid-component annealing

Content Provider	Royal Society of Chemistry (RSC)
Author	Kopielski, Andreas Csáki, Andrea Fritzsche, Wolfgang Schneider, Anne
Copyright Year	2015
Abstract	The DNA origami technique offers great potential for nanotechnology. Using biomolecular self-assembly, defined 2D and 3D nanoscale DNA structures can be realized. DNA origami allows the positioning of proteins, fluorophores or nanoparticles with an accuracy of a few nanometers and enables thereby novel nanoscale devices. Origami assembly usually includes a thermal denaturation step at 90 °C. Additional components used for nanoscale assembly (such as proteins) are often thermosensitive, and possibly damaged by such harsh conditions. They have therefore to be attached in an extra second step to avoid defects. To enable a streamlined one-step nanoscale synthesis – a so called one-pot folding – an adaptation of the folding procedures is required. Here we present a thermal optimization of this process for a 2D DNA rectangle-shaped origami resulting in an isothermal assembly protocol below 60 °C without thermal denaturation. Moreover, a room temperature protocol is presented using the chemical additive betaine, which is biocompatible in contrast to chemical denaturing approaches reported previously.
Starting Page	2102
Ending Page	2106
Page Count	5
File Format	HTM / HTML PDF
ISSN	20403364
Volume Number	7
Issue Number	5
Journal	Nanoscale
DOI	10.1039/c4nr04176c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Origami Isothermal process Self-assembly Betaine DNA Biocompatibility DNA origami Denaturation (biochemistry) Mathematical optimization Nanotechnology Three-dimensional space
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology

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