NDLI: A single-cell correlative nanoelectromechanosensing approach to detect cancerous transformation: monitoring the function of F-actin microfilaments in the modulation of the ion channel activity

Content Provider	Royal Society of Chemistry (RSC)
Author	Mashinchian, Omid Taghinejad, Mohammad Abdolahad, Mohammad Mahmoudi, Morteza Janmaleki, Mohsen Azimi, Soheil Taghinejad, Hossein Saeidi, Ali Mohajerzadeh, Shams
Copyright Year	2015
Abstract	Cancerous transformation may be dependent on correlation between electrical disruptions in the cell membrane and mechanical disruptions of cytoskeleton structures. Silicon nanotube (SiNT)-based electrical probes, as ultra-accurate signal recorders with subcellular resolution, may create many opportunities for fundamental biological research and biomedical applications. Here, we used this technology to electrically monitor cellular mechanosensing. The SiNT probe was combined with an electrically activated glass micropipette aspiration system to achieve a new cancer diagnostic technique that is based on real-time correlation between mechanical and electrical behaviour of single cells. Our studies demonstrated marked changes in the electrical response following increases in the mechanical aspiration force in healthy cells. In contrast, such responses were extremely weak for malignant cells. Confocal microscopy results showed the impact of actin microfilament remodelling on the reduction of the electrical response for aspirated cancer cells due to the significant role of actin in modulating the ion channel activity in the cell membrane.
Starting Page	1879
Ending Page	1887
Page Count	9
File Format	HTM / HTML PDF
ISSN	20403364
Volume Number	7
Issue Number	5
Journal	Nanoscale
DOI	10.1039/c4nr06102k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Cell membrane Actin Microfilament Cytoskeleton Confocal microscopy Ion Cancer
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology

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A single-cell correlative nanoelectromechanosensing approach to detect cancerous transformation: monitoring the function of F-actin microfilaments in the modulation of the ion channel activity

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