Reconfigurable pH-Responsive DNA Origami Lattices.

Content Provider	Europe PMC
Author	Julin, Sofia Linko, Veikko Kostiainen, Mauri A.
Copyright Year	2023
Abstract	DNA nanotechnology enables straightforward fabricationof user-definedand nanometer-precise templates for a cornucopia of different uses.To date, most of these DNA assemblies have been static, but dynamicstructures are increasingly coming into view. The programmabilityof DNA not only allows for encoding of the DNA object shape but alsoit may be equally used in defining the mechanism of action and thetype of stimuli-responsiveness of the dynamic structures. However,these “robotic” features of DNA nanostructures are usuallydemonstrated for only small, discrete, and device-like objects ratherthan for collectively behaving higher-order systems. Here, we showhow a large-scale, two-dimensional (2D) and pH-responsive DNA origami-basedlattice can be assembled into two different configurations (“open”and “closed” states) on a mica substrate and furtherswitched from one to the other distinct state upon a pH change ofthe surrounding solution. The control over these two configurationsis achieved by equipping the arms of the lattice-forming DNA origamiunits with “pH-latches” that form Hoogsteen-type triplexesat low pH. In short, we demonstrate how the electrostatic controlover the adhesion and mobility of the DNA origami units on the surfacecan be used both in the large lattice formation (with the help ofdirected polymerization) and in the conformational switching of thewhole lattice. To further emphasize the feasibility of the method,we also demonstrate the formation of pH-responsive 2D gold nanoparticlelattices. We believe this work can bridge the nanometer-precise DNAorigami templates and higher-order large-scale systems with the stimuli-induceddynamicity.
ISSN	19360851
Journal	ACS Nano
Volume Number	17
PubMed Central reference number	PMC10278177
Issue Number	11
PubMed reference number	37257137
e-ISSN	1936086X
DOI	10.1021/acsnano.3c03438
Language	English
Publisher	American Chemical Society
Publisher Date	2023-05-31
Access Restriction	Open
Rights License	Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). © 2023 The Authors. Published by American Chemical Society
Subject Keyword	DNA nanotechnology DNA origami metal nanoparticles DNA triplex pH control hierarchical self-assembly
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Physics and Astronomy Engineering Materials Science